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Overview
Product name
Anti-ING3 antibody
See all ING3 products (6) ...
Description
Goat polyclonal to ING3
Specificity
Tested on RKO cells transfected with ING3 (although a number of other bands were seen at similar weights). The immunogen sequence is not found in isoforms 2 and 3 listed in Q9NXR8.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Xenopus laevis
Immunogen
Synthetic peptide: CADSRSGRKSKN, corresponding to amino acids 294 - 304 of Human ING3.
CADSRSGRKS KN
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
ING3 is a tumor suppressor that acts as a potent growth regulator in normal and in established cells. p47 ING3 appears to modulate p53-mediated transcription, cell cycle control and apoptosis.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - ING3 antibody (ab3713)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 47 kDa.
Target
Relevance
ING3 belongs to a family of proteins containing the plant homeodomain (PHD) finger, which includes transcription factors and proteins that regulate chromatin structure. ING3 is a component of the NuA4 histone acetyltransferase (HAT) complex and can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of ING3 has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of the ING3 gene were detected in head and neck cancers.
Cellular localization
Nuclear
Alternative names
- 1300013A07Rik antibody
- Eaf 4 antibody
- Eaf4 antibody
see all
Database links
- Entrez Gene: 54556 Human
- Entrez Gene: 71777 Mouse
- Entrez Gene: 312154 Rat
- Omim: 607493 Human
- SwissProt: Q9NXR8 Human
- SwissProt: Q9ERB2 Mouse
- SwissProt: Q498T3 Rat
- Unigene: 489811 Human
Anti-ING3 antibody images:
Western blot - ING3 antibody (ab3713)
All lanes : Anti-ING3 antibody (ab3713) at 1/1000 dilution
Lane 1 : RKO whole cell lysate
Lane 2 : RKO cells transfected with ING3 - whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 800 conjugated Rabbit anti-Goat IgG
[H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
The identity of the non-specific bands at 33 and 20kDa has not been determined.
References for Anti-ING3 antibody (ab3713)
ab3713 has not yet been referenced specifically in any publications.
Publishing research using ab3713? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"