Anti-ING4 antibody (ab113425)
Key features and details
- Rabbit polyclonal to ING4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ING4 antibody
See all ING4 primary antibodies -
Description
Rabbit polyclonal to ING4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment, corresponding to internal sequence amino acids 33-183 of Human ING4, isoform 3 (BC007781).
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Positive control
- Human fetal liver, fetal kidney and Mouse kidney lysates; Human fetal brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Lyophilized:Reconstitute in 200ul sterile H2O. -
Storage instructions
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab113425 is purified by a protein affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa).
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IHC-P |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa). |
IHC-P
1/100 - 1/500. |
Target
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Function
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). -
Sequence similarities
Belongs to the ING family.
Contains 1 PHD-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51147 Human
- Entrez Gene: 28019 Mouse
- Entrez Gene: 297597 Rat
- Omim: 608524 Human
- SwissProt: Q9UNL4 Human
- SwissProt: Q8C0D7 Mouse
- Unigene: 524210 Human
- Unigene: 262547 Mouse
see all -
Alternative names
- Brain my036 protein antibody
- Candidate tumor suppressor p33 ING 1 homolog antibody
- Candidate tumor suppressor p33 ING1 homolog antibody
see all
Images
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All lanes : Anti-ING4 antibody (ab113425) at 1/500 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Mouse kidney lysate
Predicted band size: 26 kDa -
ab113425 at 1/100 dilution staining ING4 in Formalin-fixed, Paraffin-embedded Human fetal brain tissue by Immunohistochemistry. Note nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab113425 has been referenced in 4 publications.
- Ma T et al. Lentiviral vector with a radiation-inducible promoter, carrying the ING4 gene, mediates radiosensitization controlled by radiotherapy in cervical cancer cells. Oncol Lett 21:67 (2021). PubMed: 33365078
- Tang X et al. miR-650 promotes non-small cell lung cancer cell proliferation and invasion by targeting ING4 through Wnt-1/ß-catenin pathway. Oncol Lett 18:4621-4628 (2019). PubMed: 31611970
- Sun J et al. Inhibitor of growth 4 inhibits cell proliferation, migration, and induces apoptosis of renal cell carcinoma cells. J Cell Biochem N/A:N/A (2018). PubMed: 30390334
- Shao B & Liu E Expression of ING4 is negatively correlated with cellular proliferation and microvessel density in human glioma. Oncol Lett 14:3663-3668 (2017). PubMed: 28927128