INO80 antibody (ab22238)

Product Name 

INO80 antibody

Product type 

Primary antibodies

Description 

Mouse polyclonal to INO80

Immunogen 

Fusion protein: MDPSRETFDG STRDSYKPPN GAEMMGQSEL NTQNRIPYNT SANNRNWQIP LYWQQRGNEF QASPPPPLGY VTPEYGATGT PVNANNASRV DYATTAANVP corresponding to amino acids 1/100 of Schizosaccharomyces pombe INO80
BLAST 'MDPSRETFDG STRDSYKPPN GAEMMGQSEL NTQNRIPYNT SANNRNWQIP LYWQQRGNEF QASPPPPLGY VTPEYGATGT PVNANNASRV DYATTAANVP ' with or

Reacts with 
(species key)

S pom

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 192 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Remodeling >> INO80

Relevance 

INO80 chromatin remodeling complex is evolutionarily conserved and directly participates in the repair of a double-strand break (DSB) in yeast. INOC1/INO80 is the human homolog of S. cerevisiae INO80, the catalytic ATPase subunit of the INO80 chromatin remodeling complex. INO80/INOC1 defines a subfamily of SWI2/SNF2 chromatin remodeling proteins.

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.

A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.

Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Constituents: 50% Glycerol

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

Produced from outbred CD1 mice

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