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Anti-INO80 antibody (ab22238)

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Overview

Product name

Anti-INO80 antibody
See all INO80 products (3) ...

Description

Mouse polyclonal to INO80

Tested applications

WBmore details

Cross reactivity

Reacts with

Schizosaccharomyces pombe

Immunogen

Fusion protein: MDPSRETFDGSTRDSYKPPNGAEMMGQSELNTQNRIPYNT SANNRNWQIPLYWQQRGNEFQASPPPPLGYVTPEYGATGT PVNANNASRVDYATTAANVP, corresponding to amino acids 1/100 of Schizosaccharomyces pombe INO80

MDPSRETFDG STRDSYKPPN GAEMMGQSEL NTQNRIPYNT SANNRNWQIP LYWQQRGNEF QASPPPPLGY VTPEYGATGT PVNANNASRV DYATTAANVP

General notes

Produced from outbred CD1 mice

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Constituents: 50% Glycerol

Purity

Whole antiserum

Primary antibody notes

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Clonality

Polyclonal

Isotype

IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab22238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

Application notes

WB: 1/1000. Predicted molecular weight: 192 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

Function

DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle.

Tissue specificity

According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas.

Sequence similarities

Belongs to the SNF2/RAD54 helicase family.
Contains 1 DBINO domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.

Domain

The DBINO region is involved in binding to DNA.

Cellular localization

Nucleus. Colocalizes with PCNA at replication forks during S-phase. Recruited to DNA damage sites in a ACTR8-dependent manner.

Target information above from: UniProt accessionQ9ULG1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 4632409L19Rik antibody
  • DNA helicase INO80 antibody
  • hINO80 antibody
  • homolog of yeast INO80 antibody
  • INO80 antibody
  • INO80 complex subunit A antibody
  • INO80 homolog antibody
  • INO80_HUMAN antibody
  • INO80A antibody
  • INOC1 antibody
  • Putative DNA helicase INO80 complex homolog 1 antibody
see all

References for Anti-INO80 antibody (ab22238)

ab22238 has not yet been referenced specifically in any publications.

Publishing research using ab22238? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"