You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-INTS4 antibody (ab83452)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-INTS4 antibody
See all INTS4 products (3) ...

Description

Rabbit polyclonal to INTS4

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Cow, Dog

Immunogen

Synthetic peptide corresponding to a region within the internal amino acids 792-841 (PAEVVKILQT MLRQSAFLHL PLPEQIHKAS ATIIEPAGES DNPLRFTSGL) of human INTS4 (NP_291025).

PAEVVKILQTMLRQSAFLHLPLPEQIHKASATIIEPAGES DNPLRFTSGL

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa cell lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab83452 is purified by peptide affinity chromatography method.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> RNA polymerase
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other

  • Western blot - INTS4 antibody (ab83452)Western blot - INTS4 antibody (ab83452) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab83452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titer in peptide based assay is 1/12500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 108 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

INTS4 is a subunit of the Integrator complex, which associates with the C terminal domain of RNA polymerase II large subunit (POLR2A) and mediates 3 prime end processing of small nuclear RNAs U1 (RNU1) and U2 (RNU2) (Baillat et al., 2005 [PubMed 16239144]).

Cellular localization

Nuclear

Alternative names

  • MGC16733 antibody
  • Integrator complex subunit 4 antibody
  • MST093 antibody
  • MSTP093 antibody
  • RGD1305036 antibody
see all

Database links

Anti-INTS4 antibody images:

  Western blot - INTS4 antibody (ab83452)

Western blot - INTS4 antibody (ab83452)

Anti-INTS4 antibody (ab83452) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 108 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)


Gel concentration: 12%

References for Anti-INTS4 antibody (ab83452)

ab83452 has not yet been referenced specifically in any publications.

Publishing research using ab83452? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"