Anti-IPL-1/STK13/Aurora C antibody (ab38298)

Overview

  • Product name
    Anti-IPL-1/STK13/Aurora C antibody
    See all IPL-1/STK13/Aurora C primary antibodies
  • Description
    Rabbit polyclonal to IPL-1/STK13/Aurora C
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, selected from within amino acids 1-50 of Human IPL-1/STK13/Aurora C, (N terminal region).

  • Positive control
    • 293 cell lysates and HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ELISA 1/1000.
ICC/IF 1/100 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      May play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. May also contribute to the regulation of chromosome segregation and cytokinesis.
    • Tissue specificity
      Isoform 1 and isoform 2 are expressed in testis. Elevated expression levels were seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Expression is maximum at M phase.
    • Involvement in disease
      Defects in AURKC are the cause of male infertility with large-headed multiflagellar polyploid spermatozoa (MIMPS) [MIM:243060]; also known as infertility associated with multi-tailed spermatozoa and excessive DNA.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization
      Chromosome. Cytoplasm > cytoskeleton > spindle. Distributes in the condensed chromosomes during prophase to metaphase. After entering anaphase, there is a dissociation from separated chromosomes and a redistribution to midzone microtubules, and finally remaines in the midbody during cytokinesis.
    • Information by UniProt
    • Database links
    • Alternative names
      • AURKC antibody
      • AIE 2 antibody
      • Aie1 antibody
      • AIE2 antibody
      • AIK 3 antibody
      • AIK3 antibody
      • ARK 3 antibody
      • ARK-3 antibody
      • ARK3 antibody
      • Aur C antibody
      • AurC antibody
      • Aurkc antibody
      • AURKC_HUMAN antibody
      • aurora 3 antibody
      • Aurora C antibody
      • Aurora kinase C antibody
      • aurora related kinase 3 antibody
      • Aurora-related kinase 3 antibody
      • Aurora/Ipl1 related kinase 3 antibody
      • Aurora/Ipl1-related kinase 3 antibody
      • Aurora/Ipl1/Eg2 protein 2 antibody
      • EC 2.7.11.1 antibody
      • Serine threonine protein kinase 13 antibody
      • serine/threonine kinase 13 (aurora/IPL1 like) antibody
      • serine/threonine protein kinase aurora C antibody
      • Serine/threonine-protein kinase 13 antibody
      • Serine/threonine-protein kinase aurora-C antibody
      • SPGF5 antibody
      • STK13 antibody
      see all

    Images

    • All lanes : Anti-IPL-1/STK13/Aurora C antibody (ab38298) at 1/100 dilution

      Lane 1 : 293 cell lysate expressing Flag tag
      Lane 2 : 293 cell lysate expressing Flag tagged Aurora A
      Lane 3 : 293 cell lysate expressing Flag tagged Aurora B
      Lane 4 : 293 cell lysate expressing Flag tagged Aurora C


      Predicted band size : 36 kDa
      Observed band size : 36 kDa
      Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

      This image is courtesy of Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).

    • ICC/IF image of ab38298 at 1/100 dilution staining HeLa cells, expressing GFP-Aurora C, at different cellular mitotic stages.  The images show (A), the primary antibody (B), GFP fluorescence (C), DAPI nuclear staining and (D) ab38298 merged to DAPI staining.

    References

    ab38298 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    The localization on our datasheets is always based automatically on Protein Knowledgebase (UniProt.org). Thank you for the specification!

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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