Anti-BRAG2 antibody (ab122442)
Key features and details
- Rabbit polyclonal to BRAG2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRAG2 antibody
See all BRAG2 primary antibodies -
Description
Rabbit polyclonal to BRAG2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human BRAG2 aa 28-96 (N terminal).
Sequence:PSSETGTSLDSPSAYPQGPLVPGSSLSPDHYEHTSVGAYGLYSGPPGQQQ RTRRPKLQHSTSILRKQAE
Database link: Q6DN90 -
Positive control
- Human skin tissue; Human cell line A431
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin. -
Tissue specificity
Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis. -
Sequence similarities
Belongs to the BRAG family.
Contains 1 IQ domain.
Contains 1 PH domain.
Contains 1 SEC7 domain. -
Cellular localization
Cytoplasm. Nucleus. At steady state, may be preferentially cytosolic. - Information by UniProt
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Database links
- Entrez Gene: 9922 Human
- Omim: 610166 Human
- SwissProt: Q6DN90 Human
- Unigene: 475506 Human
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Alternative names
- ADP-ribosylation factors guanine nucleotide-exchange protein 100 antibody
- ADP-ribosylation factors guanine nucleotide-exchange protein 2 antibody
- ARF GEP100 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab122442 has been referenced in 1 publication.
- Humphreys D et al. Arf6 coordinates actin assembly through the WAVE complex, a mechanism usurped by Salmonella to invade host cells. Proc Natl Acad Sci U S A 110:16880-5 (2013). WB ; Human coronavirus . PubMed: 24085844