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Anti-IRAK antibody (ab5522)

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Overview

Product name

Anti-IRAK antibody
See all IRAK products (12) ...

Description

Rabbit polyclonal to IRAK

Tested applications

IHC-P, WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide conjugated to KLH, corresponding to amino acids 696-712 of Human IRAK.

Positive control

Jurkat cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

Research areas

Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Immunology >> Innate Immunity >> Cytokines >> Interleukins

  • Western blot - IRAK antibody (ab5522)Western blot - IRAK antibody (ab5522) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRAK antibody (ab5522)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRAK antibody (ab5522) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab5522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ShowHide1 Image

    WB

     WB: 1/100 - 1/500. Detec...Read more →

    WB: 1/100 - 1/500. Detects a band of approximately 76.5 kDa (predicted molecular weight: 76 kDa).

  • ELISA

     ELISA: 1/1000.

    ELISA: 1/1000.

Target

Function

Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.

Tissue specificity

Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain.

Post-translational
modifications

Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.

Target information above from: UniProt accessionP51617 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Il1rak antibody
  • Interleukin 1 receptor associated kinase 1 antibody
  • Interleukin 1 receptor associated kinase 2 antibody
  • Interleukin-1 receptor-associated kinase 1 antibody
  • IRAK antibody
  • IRAK-1 antibody
  • IRAK1 antibody
  • IRAK1_HUMAN antibody
  • IRAK2 antibody
  • IRAK2 antibody
  • mPLK antibody
  • OTTHUMP00000026014 antibody
  • OTTHUMP00000026015 antibody
  • OTTHUMP00000026020 antibody
  • OTTHUMP00000180621 antibody
  • Pelle antibody
  • Pelle homolog antibody
  • Pelle-like protein kinase antibody
  • Plpk antibody
see all

Database links

Anti-IRAK antibody images:

  Western blot - IRAK antibody (ab5522)

Western blot - IRAK antibody (ab5522)



Predicted band size : 76 kDa


ab5522 at a 1/100 dilution staining approximately 76.5kDa band of IRAK1 in Jurkat cell lysate by Western blot (ECL).

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRAK antibody (ab5522)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRAK antibody (ab5522)

ab5522 staining IRAK in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-IRAK antibody (ab5522)

This product has been referenced in:

  • van 't Veer C  et al. Induction of IRAK-M is associated with lipopolysaccharide tolerance in a human endotoxemia model. J Immunol 179:7110-20 (2007). WB; Human.Read more (PubMed: 17982103) »

See 1 publication for this product

Publishing research using ab5522? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"