Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Proteins and Peptides
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-IRAK4 antibody
See all IRAK4 products (9) ...
Description
Rabbit polyclonal to IRAK4
Specificity
This antibody is specific to IRAK4 and does not cross react with other IRAK proteins.
Tested applications
ICC, ICC/IF, WBmore details
Cross reactivity
Reacts with
Sheep, Human
Immunogen
Synthetic peptide corresponding to amino acids at the carboxy terminus end of IRAK-4 (Human).
Positive control
HeLa whole cell lysate.
General notes
Stable for one year.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Immunology >> Innate Immunity >> Cytokines >> Interleukins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
ICC/IF
Application notes
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa. Can be blocked with IRAK4 peptide (448-460) (ab39782).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
Involvement in disease
Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionQ9NWZ3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- IL-1 receptor-associated kinase 4 antibody
- interleukin 1 receptor associated kinase 4 mutant form 1 antibody
- Interleukin-1 receptor-associated kinase 4 antibody
see all
Database links
- Entrez Gene: 51135 Human
- Omim: 606883 Human
- SwissProt: Q69FE1 Human
- SwissProt: Q9NWZ3 Human
- Unigene: 138499 Human
Anti-IRAK4 antibody images:
Western blot - IRAK4 antibody (ab5985)
Western blot analysis of IRAK4 in HeLa cell lysate with ab5985 at 1 (lane 1), 2 (lane 2), and 4 (lane 3) µg/ml, respectively.
Immunocytochemistry/ Immunofluorescence - IRAK4 antibody (ab5985)
ab5985 at 10µg/ml staining IRAK-4 in K562 by ICC/IF
Immunocytochemistry/ Immunofluorescence - IRAK4 antibody (ab5985)
ab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.Left image: DAPI staining nuclei (blue)Middle image: IRAK4 (red)Right image: Overlay
This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview
References for Anti-IRAK4 antibody (ab5985)
This product has been referenced in:
- Kallapur SG et al. Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to chorioamnionitis. J Immunol 179:8491-9 (2007). WB; Sheep.Read more (PubMed: 18056396) »
See 1 publication for this product
Publishing research using ab5985? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"