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Product name
Anti-IRAKM antibody - Carboxyterminal end
See all IRAKM products (10) ...
Description
Rabbit polyclonal to IRAKM - Carboxyterminal end
Specificity
Anti-IRAK-M has no cross response to IRAK or IRAK2.
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Human
Immunogen
Synthetic peptide: CSSKFSWDEYEQYKKE, corresponding to C terminal amino acids 581-596 of Human IRAKM (Peptide available as ab6231 .)
CSSKFSWDEYEQYKKE
Positive control
Mouse spleen or rat kidney tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified IgG prepared by ion exchange chromatography.
Primary antibody notes
Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Immunology >> Innate Immunity >> Cytokines >> Interleukins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. PubMed: 20585389
IHC-P: Use a concentration of 2 µg/ml.
WB: Use a concentration of 0.5 - 1 µg/ml.
Target
Function
Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
Tissue specificity
Expressed predominantly in peripheral blood lymphocytes.
Involvement in disease
Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain.
Target information above from: UniProt accessionQ9Y616
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- IL 1 receptor associated kinase M antibody
- IL-1 receptor-associated kinase M antibody
- Interleukin 1 Receptor Associated Kinase 3 antibody
see all
Database links
- Entrez Gene: 11213 Human
- Entrez Gene: 73914 Mouse
- Entrez Gene: 314870 Rat
- Omim: 604459 Human
- SwissProt: Q9Y616 Human
- SwissProt: Q8K4B2 Mouse
- SwissProt: B2RYC8 Rat
- Unigene: 369265 Human
see all
Anti-IRAKM antibody - Carboxyterminal end images:
Western blot - IRAKM antibody (ab8116)
All lanes : Anti-IRAKM antibody - Carboxyterminal end (ab8116) at 1 µg/ml
Lane 1 : Mouse spleen tissue lysates
Lane 2 : Rat liver tissue lysate
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRAKM antibody (ab8116)
ab8116 at 2µg/ml staining IRAKM in rat liver cells by IHC
Immunocytochemistry/ Immunofluorescence - IRAKM antibody - Carboxyterminal end (ab8116)
ab8116 staining IRAKM in murine lung macrophages by Immunocytochemistry/ Immunofluorescence.<.br>The cells were permeablized with a 1:1 mixture of methanol and acetone. After washing with PBS, ab8116 was applied and the cells incubated for one hour. After washing, antibodies against the primary antibody labeled with FITC were applied to the cells and incubated for one hour. The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope.
Image from Lyn-Kew K et al, PLoS One. 2010 Jun 16;5(6):e11145, Fig 4.
References for Anti-IRAKM antibody - Carboxyterminal end (ab8116)
This product has been referenced in:
- Kobayashi H et al. Neutrophils activate alveolar macrophages by producing caspase-6-mediated cleavage of IL-1 receptor-associated kinase-M. J Immunol 186:403-10 (2011). WB; Human.Read more (PubMed: 21098228) »
- Villar J et al. Mechanical ventilation modulates TLR4 and IRAK-3 in a non-infectious, ventilator-induced lung injury model. Respir Res 11:27 (2010). IHC-P; Rat.Read more (PubMed: 20199666) »
See all 6 publications for this product
Publishing research using ab8116? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"