Products:Immunology >> Innate Immunity >> Cytokines >> Interferons
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab50772 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
|
|||||||||
ANSWER: |
Apologies for the attachment not coming through in the last email. I have attached file again. |
||||||||
|
|||||||||
What protocol was used to generate the IF image on the webpage? Was there an activation step? |
|||||||||
ANSWER: |
Thank you for contacting Abcam. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunofluorescent staining of HeLa cells with a 1/10 dilution of ab50772 as primary antibody and AlexaFluor®488 conjugated Goat Anti-mouse IgG diluted 1/200 as the secondary antibody
Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg
Secondary
Anti mouse IgG at 1/2500 dilution
Predicted band size : 47 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution + Ripa lysate of Hela cells at 500 µg
Secondary
Anti-mouse IgG at 1/1000 dilution
Predicted band size : 47 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution + Ripa lysate of Hela cells at 500 µg
Secondary
Anti-mouse IgG at 1/1000 dilution
1
Call 01223 696 000 or contact us