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Proteins and Peptides
ab46193
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Secondary antibodies
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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - IRF3 antibody (ab46194)
All lanes : Anti-IRF3 antibody (ab46194) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with
Lysates/proteins at 15 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - IRF3 antibody (ab46194)
ICC/IF image of ab46194 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab46194, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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