Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Innate Immunity >> Cytokines >> Interferons
Overview
Product name
Anti-IRF3 antibody
See all IRF3 products (14) ...
Description
Rabbit polyclonal to IRF3
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human IRF3.
(Peptide available as ab46193.)
Positive control
This antibody gave a positive signal in Jurkat and SHSY-5Y whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Immunology >> Innate Immunity >> Cytokines >> Interferons
Applications
Show applications key
Our Abpromise guarantee covers the use of ab46194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 51 kDa (predicted molecular weight: 47 kDa).Can be blocked with IRF3 peptide (ab46193).
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
Tissue specificity
Expressed constitutively in a variety of tissues.
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Post-translational
modifications
Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
Target information above from: UniProt accessionQ14653
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Interferon regulatory factor 3 antibody
- IRF 3 antibody
- IRF-3 antibody
see all
Database links
- Entrez Gene: 3661 Human
- GenBank: AAO24761 Human
- Omim: 603734 Human
- SwissProt: Q14653 Human
- SwissProt: Q5FBY1 Human
- SwissProt: Q5FBY2 Human
- SwissProt: Q5FBY4 Human
- Unigene: 289052 Human
see all
Anti-IRF3 antibody images:
Western blot - IRF3 antibody (ab46194)
All lanes : Anti-IRF3 antibody (ab46194) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with IRF3 peptide (ab46193) at 1 µg/ml
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with IRF3 peptide (ab46193) at 1 µg/ml
Lysates/proteins at 15 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - IRF3 antibody (ab46194)
ICC/IF image of ab46194 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab46194, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-IRF3 antibody (ab46194)
This product has been referenced in:
- Boxer ELet al. The rinderpest virus non-structural C protein blocks the induction of type 1 interferon. Virology 385:134-42 (2009). ICC/IF; Human.Read more (PubMed: 19108859) »
See 1 publication for this product
Publishing research using ab46194? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"