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Western blot - IRF3 antibody (ab76367)
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Immunocytochemistry/ Immunofluorescence - IRF3 antibody (ab76367)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF3 antibody (ab76367)
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Product Name
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IRF3 antibody
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See all IRF3 antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to IRF3
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human IRF3.
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Reacts with
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Hu
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Predicted to react
(species key)
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Chmp
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in HeLa, Jurkat and THP1 whole cell lysates.
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Cellular localization
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Cytoplasmic and Nuclear.
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Immunology >> Innate Immunity >> Cytokines >> Interferons
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Relevance
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This protein is a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IRF3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for IRF3 antibody images, references, products related to ab76367 and other tools.
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IRF3 antibody images:
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 Western blot - IRF3 antibody (ab76367)
All lanes : IRF3 antibody (ab76367) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
IRF3 contains a potential phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunocytochemistry/ Immunofluorescence - IRF3 antibody (ab76367)
ICC/IF image of ab76367 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76367, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF3 antibody (ab76367)
IHC image of IRF3 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76367, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Search PubMed (MEDLINE) for references to IRF3
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IRF3 antibody - more information
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Customer reviews (feedback) regarding IRF3 antibody |
Customer FAQs regarding IRF3 antibody |
Protocols for IRF3 antibody |
Price and availability of products related to IRF3 antibody |
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IRF3 antibody - related products:
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IRF3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about IRF3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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