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Anti-IRF5 antibody [10T1] (ab33478)

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Overview

Product name

Anti-IRF5 antibody [10T1]
See all IRF5 products (7) ...

Description

Mouse monoclonal [10T1] to IRF5

Specificity

ab33478 recognises IRF-5.

Tested applications

ICC/IF, ELISA, IP, WB, Flow Cyt, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Recombinant human IRF-5 (176-240 aa, isoform D) purified from E. coli. NCBI Accession No.: NP_001092099 (formally NP_002191).

Positive control

Extracts of Ramos, THP-1 and A20 cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

10T1

Myeloma

Sp2/0

Isotype

IgG1

Light chain type

kappa

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Helix-Turn-Helix
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol III Transcription
Immunology >> Innate Immunity >> Cytokines >> Interferons

  • Western blot - IRF5 antibody [10T1] (ab33478)Western blot - IRF5 antibody [10T1] (ab33478) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - IRF5 antibody [10T1] (ab33478)Immunocytochemistry/ Immunofluorescence - IRF5 antibody [10T1] (ab33478) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF5 antibody [10T1] (ab33478)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF5 antibody [10T1] (ab33478) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab33478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Involvement in disease

Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.

Sequence similarities

Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ13568 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Interferon regulatory factor 5 antibody
  • IRF 5 antibody
  • IRF-5 antibody
  • Irf5 antibody
  • IRF5_HUMAN antibody
  • SLEB10 antibody
see all

Database links

see all

Anti-IRF5 antibody [10T1] images:

  Western blot - IRF5 antibody [10T1] (ab33478)

Western blot - IRF5 antibody [10T1] (ab33478)

All lanes : Anti-IRF5 antibody [10T1] (ab33478) at 1/1000 dilution

Lane 1 : Ramos cell lysate
Lane 2 : THP-1 cell lysate
Lane 3 : A20 cell lysate
Lane 4 : NIH 3T3 cell lysate

Secondary
goat anti-mouse secondary antibody conjugated to HRP
developed using the ECL technique

Predicted band size : 58 kDa
Observed band size : 58 kDa
Additional bands at : 26 kDa,80 kDa. We are unsure as to the identity of these extra bands.

100-200µg of whole cell lysates were used for western blotting.

  Immunocytochemistry/ Immunofluorescence - IRF5 antibody [10T1] (ab33478)

Immunocytochemistry/ Immunofluorescence - IRF5 antibody [10T1] (ab33478)

Immunofluorescence/Immunocytochemistry analysis of HEK293 cells (105-106) stained with Mouse monoclonal [10T1] to IRF5 (ab33478 1/100-1/200).

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF5 antibody [10T1] (ab33478)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF5 antibody [10T1] (ab33478)

ab33478 at 1/50 dilution staining IRF5 in Human normal colon mucosa tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The section was incubated with the primary antibody for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF5 antibody [10T1] (ab33478)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF5 antibody [10T1] (ab33478)

ab33478 at 1/50 dilution staining IRF5 in Human colon adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The section was incubated with the primary antibody for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

  Flow Cytometry-Anti-IRF5 antibody [10T1](ab33478)

Flow Cytometry-Anti-IRF5 antibody [10T1](ab33478)

Overlay histogram showing THP1 cells stained with ab33478 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33478, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-IRF5 antibody [10T1] (ab33478)

This product has been referenced in:

  • Pandey AK  et al. NOD2, RIP2 and IRF5 play a critical role in the type I interferon response to Mycobacterium tuberculosis. PLoS Pathog 5:e1000500 (2009). WB; Mouse.Read more (PubMed: 19578435) »
  • Watanabe T  et al. Muramyl dipeptide activation of nucleotide-binding oligomerization domain 2 protects mice from experimental colitis. J Clin Invest 118:545-59 (2008). WB; Human.Read more (PubMed: 18188453) »

See all 4 publications for this product

Publishing research using ab33478? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"