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- Proteins and Peptides
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Product name
Anti-IRF5 antibody
See all IRF5 products (7) ...
Description
Rabbit polyclonal to IRF5
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow
Immunogen
Synthetic peptide conjugated to BSA derived from within residues 450 to the C-terminus of Human IRF5.
(Peptide available as ab224 11.)
Positive control
This antibody gave a positive signal in the following lysates: HeLa (Human epithelial carcinoma cell line) whole cell Liver (Mouse) Tissue Kidney (Mouse) Tissue Liver (Rat) Tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Helix-Turn-Helix
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol III Transcription
Immunology >> Innate Immunity >> Cytokines >> Interferons
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).Can be blocked with IRF5 peptide (ab22411).
Application notes
Is unsuitable for or IHC-P.
Target
Involvement in disease
Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ13568
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Interferon regulatory factor 5 antibody
- IRF 5 antibody
- IRF-5 antibody
see all
Database links
- Entrez Gene: 615340 Cow
- Entrez Gene: 3663 Human
- Entrez Gene: 27056 Mouse
- Entrez Gene: 296953 Rat
- Omim: 607218 Human
- SwissProt: Q58DJ0 Cow
- SwissProt: Q64GB0 Human
- SwissProt: Q64GA9 Human
see all
Anti-IRF5 antibody images:
Western blot - IRF5 antibody (ab21689)
All lanes : Anti-IRF5 antibody (ab21689) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to IgG (700) at 1/10000 dilution
Predicted band size : 58 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Western blot - IRF5 antibody (ab21689)
All lanes : Anti-IRF5 antibody (ab21689) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
References for Anti-IRF5 antibody (ab21689)
This product has been referenced in:
- Krausgruber T et al. IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses. Nat Immunol 12:231-8 (2011). WB; Mouse.Read more (PubMed: 21240265) »
- Li Q & Tainsky MA Epigenetic Silencing of IRF7 and/or IRF5 in Lung Cancer Cells Leads to Increased Sensitivity to Oncolytic Viruses. PLoS One 6:e28683 (2011). WB; Human.Read more (PubMed: 22194884) »
See all 3 publications for this product
Publishing research using ab21689? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"