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Anti-IRF6 antibody (ab10925)

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Overview

Product name

Anti-IRF6 antibody
See all IRF6 products (8) ...

Description

Goat polyclonal to IRF6

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: C-TPSMQLPPALPPQ, corresponding to C terminal amino acids 455-467 of Human IRF6(Peptide available as ab232 04.)

C-TPSMQLPPALPPQ

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human Ovary lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Embryonic Stem Cells >> Cytokines
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Helix-Turn-Helix
Immunology >> Innate Immunity >> Cytokines >> Interferons

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     

  • WB

     

Application notes

ELISA: 1/32000.
WB: Use at a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa) in Human Ovary lysate.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development (By similarity). Plays a role in regulating mammary epithelial cell proliferation.

Tissue specificity

Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas.

Involvement in disease

Defects in IRF6 are a cause of van der Woude syndrome (VWS) [MIM:119300]; also known as lip-pit syndrome (LPS). It is an autosomal dominant developmental disorder characterized by lower lip pits, cleft lip and/or cleft palate. Penetrance is incomplete. Van der Woude and popliteal pterygium syndrome are allelic disorders.
Defects in IRF6 are the cause of popliteal pterygium syndrome (PPS) [MIM:119500]. PPS is an autosomal dominant developmental disorder characterized by cleft lip and/or cleft palate, and skin and genital anomalies. Penetrance is incomplete and expressivity is variable. It shows orofacial phenotypic similarities with van der Woude syndrome. Van der Woude and popliteal pterygium syndrome are allelic disorders.
Genetic variation in IRF6 is associated with non-syndromic orofacial cleft type 6 (OFC6) [MIM:608864]; also called non-syndromic cleft lip with or without cleft palate 6. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.

Sequence similarities

Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Post-translational
modifications

Phosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation.

Cellular localization

Nucleus. Cytoplasm. Translocates to nucleus in response to an activating signal.

Target information above from: UniProt accessionO14896 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Interferon regulatory factor 6 antibody
  • IRF 6 antibody
  • IRF-6 antibody
  • IRF6 antibody
  • IRF6_HUMAN antibody
  • LPS antibody
  • OFC 6 antibody
  • OFC6 antibody
  • OTTHUMP00000034677 antibody
  • OTTHUMP00000034678 antibody
  • PIT antibody
  • PPS antibody
  • VWS antibody
  • VWS1 antibody
see all

Database links

References for Anti-IRF6 antibody (ab10925)

ab10925 has not yet been referenced specifically in any publications.

Publishing research using ab10925? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"