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Anti-IRF8 antibody (ab28696) at 1.25 µg/ml + Jurkat cell lysate
Secondary
HRP conjugated anti-Rabbit IgG
Predicted band size : 47 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
Western blot with ab28696 at 1.25µg/ml using HRP conjugated anti-Rabbit IgG diluted in 1/50000 - 1/100000 as secondary antibody and jurkat cell lysate at a gel concentration of 12%.
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) using ab28696 using ab 28696 and human kidney tissue.
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