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Overview
Product name
Anti-IRF8 antibody
See all IRF8 products (6) ...
Description
Rabbit polyclonal to IRF8
Specificity
ab28696 recognises Interferon regulatory factor 8 (IRF8).
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide derived from a region between amino acids 43-92 of human ICSBP. (Note: The exact amino acid sequence is proprietary).
Positive control
Jurkat cell lysate; Human kidney slide
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlPredicted molecular weight: 47 kDa.
IHC-P: Use a concentration of 4 - 8 µg/ml.
ELISA
ELISA: 1/62500ELISA: 1/62500
Target
Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
Tissue specificity
Predominantly in lymphoid tissues.
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Post-translational
modifications
Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ02556
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- H ICSBP antibody
- H-ICSBP antibody
- HGNC:5358 antibody
see all
Database links
- Entrez Gene: 3394 Human
- Entrez Gene: 15900 Mouse
- Entrez Gene: 292060 Rat
- Omim: 601565 Human
- SwissProt: Q02556 Human
- SwissProt: P23611 Mouse
- SwissProt: Q3TZQ3 Mouse
- SwissProt: Q3U068 Mouse
see all
Anti-IRF8 antibody images:
Western blot - ICSBP antibody (ab28696)
Anti-IRF8 antibody (ab28696) at 1.25 µg/ml + Jurkat cell lysate
Secondary
HRP conjugated anti-Rabbit IgG
Predicted band size : 47 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
Western blot with ab28696 at 1.25µg/ml using HRP conjugated anti-Rabbit IgG diluted in 1/50000 - 1/100000 as secondary antibody and jurkat cell lysate at a gel concentration of 12%.
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - ICSBP antibody (ab28696)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) using ab28696 using ab 28696 and human kidney tissue.
References for Anti-IRF8 antibody (ab28696)
ab28696 has not yet been referenced specifically in any publications.
Publishing research using ab28696? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"