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Overview
Product name
Anti-IRF8 antibody
See all IRF8 products (6) ...
Description
Goat polyclonal to IRF8
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: QRSFFRENQQITV, corresponding to C terminal amino acids 414-426 of Human ICSBP.
C-QRSFFREN QQITV
Positive control
K562 cell lysate
General notes
Principal Names – ICSBP1; IRF8; ICSBP; IRF-8; interferon consensus sequence binding protein 1; H-ICSBP; interferon regulatory factor 8.
Official Gene Symbol - ICSBP1.
GenBank Accession Number – NP_002154.
LocusLink ID - 3394 (human); 15900 (mouse).
Gene Ontology terms - RNA polymerase II transcription factor activity; RNA polymerase II transcription factor activity, enhancer binding; immune response; negative regulation of transcription from Pol II promoter.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
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Western blot - IRF8 antibody (ab3428)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 47 kDa in K562 lysate(predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
Tissue specificity
Predominantly in lymphoid tissues.
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Post-translational
modifications
Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ02556
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- H ICSBP antibody
- H-ICSBP antibody
- HGNC:5358 antibody
see all
Database links
- Entrez Gene: 3394 Human
- Entrez Gene: 15900 Mouse
- Entrez Gene: 292060 Rat
- Omim: 601565 Human
- SwissProt: Q02556 Human
- SwissProt: P23611 Mouse
- SwissProt: Q3TZQ3 Mouse
- SwissProt: Q3U068 Mouse
see all
Anti-IRF8 antibody images:
Western blot - IRF8 antibody (ab3428)
Predicted band size : 47 kDa
ab3428 at a 1µg/ml concentration staining ICSBP in K562 lysate (RIPA buffer, 30 µg total protein per lane, 1 hour incubation). Detected by Western blot (ECL).
References for Anti-IRF8 antibody (ab3428)
ab3428 has not yet been referenced specifically in any publications.
Publishing research using ab3428? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"