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Anti-IRS1 antibody [EP263Y] (ab40777)

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Overview

Product name

Anti-IRS1 antibody [EP263Y]
See all IRS1 products (44) ...

Description

Rabbit monoclonal [EP263Y] to IRS1

Tested applications

WB, ICC/IF, IP, IHC-Pmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

Synthetic peptide corresponding to residues near the C-terminus of human IRS-1.

Positive control

HeLa cell lysate, Human breast carcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP263Y

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Obesity
Metabolism >> Types of disease >> Diabetes
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cancer >> Signal transduction >> Other
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Adapters >> Cytoplasmic
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

  • Western blot - IRS1 antibody [EP263Y] (ab40777)Western blot - IRS1 antibody [EP263Y] (ab40777) image (enlarge)

  • - IRS1 antibody [EP263Y] (ab40777)- IRS1 antibody [EP263Y] (ab40777) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab40777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/250 - 1/500.
IHC-P: 1/50 - 1/100.
IP: 1/50.
WB: 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa).

Is unsuitable for FACS.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

Involvement in disease

Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

Sequence similarities

Contains 1 IRS-type PTB domain.
Contains 1 PH domain.

Post-translational
modifications

Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding.

Target information above from: UniProt accessionP35568 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • HIRS 1 antibody
  • HIRS1 antibody
  • Insulin Receptor Substrate 1 antibody
  • IRS 1 antibody
  • IRS-1 antibody
  • IRS1 antibody
  • IRS1_HUMAN antibody
  • OTTHUMP00000164234 antibody
see all

Database links

Anti-IRS1 antibody [EP263Y] images:

  Western blot - IRS1 antibody [EP263Y] (ab40777)

Western blot - IRS1 antibody [EP263Y] (ab40777)

Anti-IRS1 antibody [EP263Y] (ab40777) at 1/1000 dilution + HeLa cell lysate at 10 µg

Predicted band size : 170 kDa
Observed band size : 170 kDa

  - IRS1 antibody [EP263Y] (ab40777)

- IRS1 antibody [EP263Y] (ab40777)

ab40777, staining human breast carcinoma by immunohistochemistry, paraffin-embedded tissue.

References for Anti-IRS1 antibody [EP263Y] (ab40777)

ab40777 has not yet been referenced specifically in any publications.

Publishing research using ab40777? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"