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- Secondary antibodies
- Lysates
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Product name
Anti-IRS1 antibody
See all IRS1 products (44) ...
Description
Rabbit polyclonal to IRS1
Specificity
This antibody is specific for human IRS 1. By immunoblot analysis, an immunoreactive protein was detected from 3T3 cell lysate. This band is abolished by the depletion of the synthetic peptide.
Tested applications
WB, IP, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: ITATSPAS, corresponding to amino acids 308-315 of Human IRS 1. In Mouse and Rat, the synthetic peptide is a portion of amino acid 303 - 310.
ITATSPAS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Obesity
Metabolism >> Types of disease >> Diabetes
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cancer >> Signal transduction >> Other
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Adapters >> Cytoplasmic
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
ELISA
Application notes
ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
IP: Use 3.0-5.0µg for 107 cells.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Predicted molecular weight: 200 kDa in cell lysates derived from 3T3 cells, which corresponds to the predicted molecular weight of IRS 1. Samples were boiled prior to subject 7.5% SDS polyacrylamide gel electrophoresis.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Involvement in disease
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Sequence similarities
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
Post-translational
modifications
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding.
Target information above from: UniProt accessionP35568
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HIRS 1 antibody
- HIRS1 antibody
- Insulin Receptor Substrate 1 antibody
see all
Database links
- Entrez Gene: 3667 Human
- GenBank: NM_005544 Human
- Omim: 147545 Human
- SwissProt: P35568 Human
- Unigene: 471508 Human
References for Anti-IRS1 antibody (ab3866)
ab3866 has not yet been referenced specifically in any publications.
Publishing research using ab3866? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"