The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204853 (IRS1 peptide) can be utilized as a substrate for the following active protein kinases:
ab64298 (Active human Axl protein fragment) ab85755 (Active human Dystrophia myotonica protein kinase full length protein) ab69988 (Active Insulin Receptor R protein fragment) ab70687 (Active human Insulin Receptor protein fragment) ab70541 (Active human RON protein fragment) ab60337 (Active human Serine/threonine-protein kinase 4 full length protein) ab55710 (Active human LOK protein fragment)
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Dilute peptide in distilled water to a final concentration of 1mg/ml
Insulin receptor substrate 1
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Involvement in disease
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding.