Recombinant Anti-IRS1 antibody [EP260Y] (ab46800)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP260Y] to IRS1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IRS1 antibody [EP260Y]
See all IRS1 primary antibodies -
Description
Rabbit monoclonal [EP260Y] to IRS1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged human IRS1 recombinant protein (aa801-1020)
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General notes
This antibody was re-validated and was confirmed to recognize total IRS1. The name was changed from Anti-IRS1 (phospho Y896) antibody [EP260Y] to Anti-IRS1 antibody [EP260Y] on May 1st, 2018.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP260Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000. Predicted molecular weight: 27 kDa.
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Notes |
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WB
1/10000. Predicted molecular weight: 27 kDa. |
Target
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Function
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
Involvement in disease
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
Sequence similarities
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
Post-translational
modificationsSerine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
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Database links
- Entrez Gene: 3667 Human
- Omim: 147545 Human
- SwissProt: P35568 Human
- Unigene: 471508 Human
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Alternative names
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
Images
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Anti-IRS1 antibody [EP260Y] (ab46800) at 1/10000 dilution + His-tagged human IRS1 recombinant protein (aa801-1020), 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Exposure time: 1 secondBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Primary and secondary antibodies incubated for 1hr at room temperature.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab46800 has been referenced in 16 publications.
- Zhao J et al. CircCCDC91 regulates chicken skeletal muscle development by sponging miR-15 family via activating IGF1-PI3K/AKT signaling pathway. Poult Sci 101:101803 (2022). PubMed: 35334442
- Peng L et al. Amelioration of Hippocampal Insulin Resistance Reduces Tau Hyperphosphorylation and Cognitive Decline Induced by Isoflurane in Mice. Front Aging Neurosci 13:686506 (2021). PubMed: 34512303
- Li L et al. Mesenchymal stromal cells protect hepatocytes from lipotoxicity through alleviation of endoplasmic reticulum stress by restoring SERCA activity. J Cell Mol Med 25:2976-2993 (2021). PubMed: 33591626
- Rivera P et al. Perinatal free-choice of a high-calorie low-protein diet affects leptin signaling through IRS1 and AMPK dephosphorylation in the hypothalami of female rat offspring in adulthood. Acta Physiol (Oxf) 226:e13244 (2019). PubMed: 30589509
- Zhou D et al. Sodium butyrate reduces high-fat diet-induced non-alcoholic steatohepatitis through upregulation of hepatic GLP-1R expression. Exp Mol Med 50:157 (2018). PubMed: 30510243