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- Secondary antibodies
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Product name
Anti-IRS2 (phospho S731) antibody
See all IRS2 products (6) ...
Description
Rabbit polyclonal to IRS2 (phospho S731)
Specificity
This antibody is specific for human IRS2 phosphorylated at the position of Serine 731. The antibodies were evaluated for specificity with a dot blot assay using synthetically prepared IRS peptides. It only recognizes the phosphorylated serine 731, not other phosphorylated sites or non-phosphorylated IRS 1 or IRS 2.
Tested applications
WB, IP, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide (YKASSpPAESS), corresponding to amino acids 727-736 of human IRS2 at the phosphorylated site of Serine 731. This synthetic peptide also correlates to amino acids 719-728 of mouse IRS2 at the phosphorylation site of Serine 723 and to amino acids 229-238 of rat IRS2 at the serine phosphorylation site 233.
YKASSPAESS
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Diabetes
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Cardiovascular >> Atherosclerosis >> Diabetes associated
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 200 kDa.in cell lysate derived from mouse serum-treated fibroblasts, which corresponds to the predicted molecular weight of phospho IRS 2. Samples were boiled prior to subject 7.5% SDS-polyacrylamide gelelectrophoresis.
IP: Use at an assay dependent dilution. Use at a concentration of 3.0 - 5.0 µg / extract from 107 cells.
ELISA: Use a concentration of 0.1 - 1 µg/ml.
IHC-P: Use a concentration of 2 µg/ml.
Target
Function
May mediate the control of various cellular processes by insulin.
Sequence similarities
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm > cytosol.
Target information above from: UniProt accessionQ9Y4H2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Insulin Receptor Substrate 2 antibody
- Insulin Receptor Substrate 2 antibody
- IRS 2 antibody
see all
Database links
- Entrez Gene: 8660 Human
- Entrez Gene: 384783 Mouse
- Omim: 600797 Human
- SwissProt: Q9Y4H2 Human
- SwissProt: P81122 Mouse
- Unigene: 442344 Human
- Unigene: 407207 Mouse
Anti-IRS2 (phospho S731) antibody images:
Immunoprecipitation - IRS2 (phospho S731) antibody (ab3690)
IP: 3T3 cell lysate immunoprecipitated with Anti-IRS-2 (pSER731) (ab3690)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-IRS2 (phospho S731) antibody(ab3690)
ab3690 (2µg/ml) staining IRS2 (phospho S731) in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cell membrane.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - IRS2 (phospho S731) antibody (ab3690)
Anti-IRS2 (phospho S731) antibody (ab3690) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
Additional bands at : 121 kDa,64 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
IRS2 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 160 kDa band observed is also comparable to the molecular weight seen with other commercially available antibodies to IRS2.
References for Anti-IRS2 (phospho S731) antibody (ab3690)
This product has been referenced in:
- Huang J et al. Iron overload and diabetes risk: a shift from glucose to Fatty Acid oxidation and increased hepatic glucose production in a mouse model of hereditary hemochromatosis. Diabetes 60:80-7 (2011). WB; Mouse.Read more (PubMed: 20876715) »
- Enjyoji K et al. Deletion of cd39/entpd1 results in hepatic insulin resistance. Diabetes 57:2311-20 (2008).Read more (PubMed: 18567823) »
See all 2 publications for this product
Publishing research using ab3690? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"