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- Secondary antibodies
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Product name
Anti-ITM2B antibody
See all ITM2B products (8) ...
Description
Chicken polyclonal to ITM2B
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: MVKVTFNSAL AQKEAKKDEP KSGEEALIIP PDAVAVDCKD PDDVVPVGQR RAWCWCMCFG, corresponding to amino acids 1-60 of ITM2B.
MVKVTFNSAL AQKEAKKDEP KSGEEALIIP PDAVAVD CKD PDDVVPVGQR RAWCWCMCFG
Positive control
E Coli-derived fusion protein.
General notes
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Sensory System >> Auditory system
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Western blot - ITM2B antibody (ab14307)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Predicted molecular weight: 32 kDa.
IHC-P: Use at an assay dependent dilution. See Abreview.
Target
Function
Functions as a protease inhibitor. Plays a role in APP processing regulating the physiological production of the beta amyloid peptide. Restricts docking of gamma-secretase to APP and access of alpha- and beta-secretase to their cleavage APP sequence.
Tissue specificity
Expressed in brain and in other tissues.
Involvement in disease
Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 1 (CAA-ITM2B1) [MIM:176500]. A disorder characterized by amyloid deposition in the walls of cerebral blood vessels and neurodegeneration in the central nervous system. Cerebral amyloid angiopathy, non-neuritic and perivascular plaques and neurofibrillary tangles are the predominant pathological lesions. Clinical features include progressive mental deterioration, spasticity and muscular rigidity.
Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 2 (CAA-ITM2B2) [MIM:117300]; also known as heredopathia ophthalmo-oto-encephalica. A disorder characterized by amyloid deposition in the walls of the blood vessels of the cerebrum, choroid plexus, cerebellum, spinal cord and retina. Plaques and neurofibrillary tangles are observed in the hippocampus. Clinical features include progressive ataxia, dementia, cataracts and deafness.
Sequence similarities
Belongs to the ITM2 family.
Contains 1 BRICHOS domain.
Post-translational
modifications
The C-terminal part of the ectodomain is processed by furin and related proteases producing a secreted peptide of 4 to 5 kDa. For the ABRI and ADAN variants the C-terminal secreted peptide is larger and may produce amyloid fibrils responsible for neuronal dysfunction and dementia. The remaining part of the ectodomain containing the BRICHOS domain is cleaved by ADAM10 and is secreted as a peptide of 25 kDa. The membrane-bound N-terminal fragment (NTF) of 22 kDa is further proteolytically processed by SPPL2A and SPPL2B through regulated intramembrane proteolysis producing a secreted peptide (BRI2C) and an intracellular domain (ICD) released in the cytosol.
Cellular localization
Golgi apparatus membrane. Cell membrane.
Target information above from: UniProt accessionQ9Y287
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABRI antibody
- ABri/ADan amyloid peptide antibody
- BRI 2 antibody
see all
Database links
- Entrez Gene: 9445 Human
- Entrez Gene: 16432 Mouse
- Entrez Gene: 290364 Rat
- Omim: 603904 Human
- SwissProt: Q9Y287 Human
- SwissProt: O89051 Mouse
- SwissProt: Q5XIE8 Rat
- Unigene: 643683 Human
see all
Anti-ITM2B antibody images:
Western blot - ITM2B antibody (ab14307)
Anti-ITM2B antibody (ab14307) at 1/2000 dilution + E.coli derived ITM2B fusion protein
Secondary
HRP conjugated goat anti IgY at 1/1000 dilution
Predicted band size : 32 kDa
References for Anti-ITM2B antibody (ab14307)
This product has been referenced in:
- Han C et al. Identification of heat shock protein 5, calnexin and integral membrane protein 2B as Adam7-interacting membrane proteins in mouse sperm. J Cell Physiol 226:1186-95 (2011). WB, IP; Mouse.Read more (PubMed: 20945367) »
- Kilger E et al. BRI2 Protein Regulates {beta}-Amyloid Degradation by Increasing Levels of Secreted Insulin-degrading Enzyme (IDE). J Biol Chem 286:37446-57 (2011). WB; Human.Read more (PubMed: 21873424) »
See all 4 publications for this product
Publishing research using ab14307? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"