Anti-BRI3 antibody (ab101389)
Key features and details
- Rabbit polyclonal to BRI3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRI3 antibody -
Description
Rabbit polyclonal to BRI3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human BRI3 aa 110-258.
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Positive control
- HeLa, HepG2, MCF7 cell lines.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (PH8.0)
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 30 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (PH8.0) |
Target
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Function
Negative regulator of beta amyloid peptide production. May inhibit the processing of APP by blocking its access to alpha-and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation. -
Tissue specificity
High levels in the brain, specifically in the cerebral cortex, medulla, amygdala, hippocampus, thalamus, caudate nucleus, cerebellum, olfactory lobe and spinal cord. Very low levels in other organs. -
Sequence similarities
Belongs to the ITM2 family.
Contains 1 BRICHOS domain. -
Post-translational
modificationsType I membrane-bound, as well as soluble, furin has a pre-eminent role in ITM2C proteolytic processing. PCSK7 and PCSK5 may also be involved although to a lesser extent. The soluble form of PCSK7 is incapable of processing ITM2C. -
Cellular localization
Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 281256 Cow
- Entrez Gene: 539959 Cow
- Entrez Gene: 81618 Human
- Entrez Gene: 64294 Mouse
- Entrez Gene: 100144481 Pig
- Omim: 609554 Human
- SwissProt: A2VDN0 Cow
- SwissProt: Q9NQX7 Human
see all -
Alternative names
- BRI3 antibody
- BRICD2C antibody
- BRICHOS domain containing 2C antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab101389 has been referenced in 2 publications.
- Yasukawa T et al. NRBP1-Containing CRL2/CRL4A Regulates Amyloid ß Production by Targeting BRI2 and BRI3 for Degradation. Cell Rep 30:3478-3491.e6 (2020). PubMed: 32160551
- Dolfe L et al. The Bri2 and Bri3 BRICHOS Domains Interact Differently with Aß42 and Alzheimer Amyloid Plaques. J Alzheimers Dis Rep 2:27-39 (2018). PubMed: 30480246