Anti-ITPR2 antibody (ab5805)
Key features and details
- Rabbit polyclonal to ITPR2
- Suitable for: ICC, IHC-Fr
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ITPR2 antibody
See all ITPR2 primary antibodies -
Description
Rabbit polyclonal to ITPR2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Frmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide corresponding to Rat ITPR2 aa 317-334.
Sequence:ELNPDYRDAQNEGKTVRD
(Peptide available asab5842) -
Positive control
- IHC-Fr: Rat brain tissue. ICC: SH-SY5Y cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
Use a concentration of 2 µg/ml.
|
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IHC-Fr |
1/100.
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Notes |
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ICC
Use a concentration of 2 µg/ml. |
IHC-Fr
1/100. |
Target
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Function
Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA. -
Tissue specificity
Isoform Short is found in skeletal muscle and heart. -
Involvement in disease
Anhidrosis, isolated, with normal sweat glands -
Sequence similarities
Belongs to the InsP3 receptor family.
Contains 5 MIR domains. -
Domain
The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. -
Post-translational
modificationsPhosphorylation by cAMP-dependent PKA on Ser-937 increases calcium release. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 281878 Cow
- Entrez Gene: 3709 Human
- Entrez Gene: 81678 Rat
- Omim: 600144 Human
- SwissProt: Q8WN96 Cow
- SwissProt: Q14571 Human
- SwissProt: P29995 Rat
- Unigene: 512235 Human
see all -
Alternative names
- 4 antibody
- 5-trisphosphate receptor antibody
- Inositol 1 4 5 trisphosphate receptor type 2 antibody
see all
Images
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4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling ITPR2 using ab5805 at 2 μg/ml in 0.1% BSAfor 3 hours at RT, followed by a Goat Anti-Rabbit (H&L) AlexaFluor® secondary antibody (Green (a)). The nuclear counter stain is DAPI (Blue (b)). F-actin is stained with AlexaFluor® 555-Rhodamine (Red (c)). Panel D shows the merged image. Panel (e) is a secondary antibody only control.
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Shows immunolocalization of IP3R-II in rat brain using ab5805.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab5805 has been referenced in 4 publications.
- Jaiswal PK et al. Biochemical characteristics of the chondrocyte-enriched SNORC protein and its transcriptional regulation by SOX9. Sci Rep 10:7790 (2020). PubMed: 32385306
- Causton B et al. CARMA3 Mediates Allergic Lung Inflammation in Response to Alternaria alternata. Am J Respir Cell Mol Biol 59:684-694 (2018). PubMed: 29958012
- Kapoor N et al. Regulation of calcium clock-mediated pacemaking by inositol-1,4,5-trisphosphate receptors in mouse sinoatrial nodal cells. J Physiol 593:2649-63 (2015). PubMed: 25903031
- Okamoto Y et al. Arrhythmogenic coupling between the Na+ -Ca2+ exchanger and inositol 1,4,5-triphosphate receptor in rat pulmonary vein cardiomyocytes. J Mol Cell Cardiol 52:988-97 (2012). ICC/IF ; Rat . PubMed: 22285480