Anti-Iba1 antibody [1022-5] - Microglial marker (ab15690)

Overview

• Product nameAnti-Iba1 antibody [1022-5] - Microglial markerSee all Iba1 primary antibodies ...
• Description
  Mouse monoclonal [1022-5] to Iba1 - Microglial marker
• SpecificitySome customers have reported successful staining using this antibody on frozen sections, however Ab15690 is not routinely tested on frozen sections, and we do not recommend this antibody for use with frozen tissue sections.
• Tested applicationsFlow Cyt, ICC/IF, IHC-FoFr, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Recombinant full length protein (Human).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.2
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number1022-5
• IsotypeIgG2b
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Immunology
  • Immune Interventions
  • Transplantation

  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Microglia marker

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Other

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• FunctionActin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
• Tissue specificityDetected in T-lymphocytes and peripheral blood mononuclear cells.
• Sequence similaritiesContains 2 EF-hand domains.
• Post-translational
  modificationsPhosphorylated on serine residues.
• Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

Target information above from: UniProt accession P55008 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 199 Human
  • Entrez Gene: 11629 Mouse
  • Entrez Gene: 29427 Rat
  • Omim: 601833 Human
  • SwissProt: P55008 Human
  • SwissProt: O70200 Mouse
  • SwissProt: P55009 Rat
  • Unigene: 76364 Human

  • Unigene: 10747 Mouse
  • Unigene: 32080 Rat

  see all

• Alternative names
  AIF 1 antibodyAIF-1 antibodyAIF1 antibody

  AIF1 protein antibodyAIF1_HUMAN antibodyallograft inflammatory factor 1 antibodyAllograft inflammatory factor 1 splice variant G antibodyallograft inflammatory factor-1 splice variant Hara-1 antibodyballoon angioplasty responsive transcription antibodyBART 1 antibodyG1 antibodyG1 putative splice variant of allograft inflamatory factor 1 antibodyIBA 1 antibodyIBA1 antibodyinterferon gamma responsive transcript antibodyinterferon responsive transcript factor 1 antibodyIonized calcium binding adapter molecule 1 antibodyIonized calcium-binding adapter molecule 1 antibodyionized calcium-binding adapter molecule antibodyIRT 1 antibodyIRT1 antibodyMicroglia response factor antibodyMRF1 antibodyProtein G1 antibody

  see all

Anti-Iba1 antibody [1022-5] - Microglial marker images

• 

  Immunocytochemistry/ Immunofluorescence - Iba1 antibody [1022-5] - Microglial marker (ab15690)This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemy

  ab15690 at 1/50 staining rat primary cortical cells. The cells were formaldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An Alexa-Fluor ® 488 conjugated goat anti-mouse antibody was used as the secondary.

  See Abreview

• 

  Flow Cytometry-Iba1 antibody [1022-5] - Microglial marker(ab15690)
  Overlay histogram showing RAW 264.7 cells stained with ab15690 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab15690, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  
• 

  Western blot - Anti-Iba1 antibody [1022-5] - Microglial marker (ab15690)
  Anti-Iba1 antibody [1022-5] - Microglial marker (ab15690) at 1/750 dilution + Mouse microglial whole cell lysate at 35 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97023) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 1 minute

  This image is courtesy of an anonymous Abreview

  See Abreview