- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Iba1 antibody
See all Iba1 primary antibodies -
DescriptionGoat polyclonal to Iba1
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Host speciesGoat
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SpecificityThis antibody is expected to recognise isoform 3 (NP_001614.3) only. This antibody gave excellent staining in paraffin embedded human adult tissue and embyronic rat tissue. For WB, we recommend blocking in 3-5% milk, NOT BSA. Blocking in BSA gives high background.
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Tested applicationsSuitable for: WB, IHC-Fr, IHC-Pmore details
Unsuitable for: IHC-FoFr -
Species reactivityReacts with: Mouse, Rabbit, Human
Predicted to work with: Cow, Pig, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human Iba1 aa 28-42 (internal sequence) (Cysteine residue). NP_001614.3
Sequence:C-NKQFLDDPKYSSDED
Database link: P55008 -
Positive control
- WB: Human peripheral blood mononucleocyte and frontal cortex lysate. IHC-P: Mouse brain; Human placenta and jejunum; rat embryonic liver tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab48004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). We recommend blocking in 3-5% milk, not BSA. Blocking in BSA gives high background in WB. |
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| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionActin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
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Tissue specificityDetected in T-lymphocytes and peripheral blood mononuclear cells.
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Sequence similaritiesContains 2 EF-hand domains.
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Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
- Information by UniProt
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Database links
- Entrez Gene: 280989 Cow
- Entrez Gene: 199 Human
- Entrez Gene: 11629 Mouse
- Entrez Gene: 397271 Pig
- Omim: 601833 Human
- SwissProt: Q9BDK2 Cow
- SwissProt: P55008 Human
- SwissProt: O70200 Mouse
see all -
Alternative names
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab48004)Paraffin embedded mouse brain tissue stained for Iba1 with ab48004 (2 µg/ml) in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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Western blot - Anti-Iba1 antibody (ab48004)Anti-Iba1 antibody (ab48004) at 0.5 µg/ml + human frontal cortex lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 17 kDaPrimary incubation was 1 hour
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Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody (ab48004)Paraformaldehyde fixed mouse brain tissue stained for Iba1 with ab48004 (1/200 dilution) in immunohistochemical analysis. Secondary: Donkey Anti-Goat IgG Cy3® (1/500 dilution).
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Western blot - Anti-Iba1 antibody (ab48004)All lanes : Anti-Iba1 antibody (ab48004) at 1 µg/ml
Lane 1 : Human peripheral blood mononucleocyte lysate in RIPA buffer
Lane 2 : Human peripheral blood mononucleocyte lysate in RIPA buffer with blocking peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Additional bands at: 110 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
1 hour primary antibody incubation. Additional bands of unknown identity observed at 35kDa and 110kDa. These bands were successfully blocked by incubation with the immunising peptide -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab48004)This image is courtesy of an Abreview submitted by Mr Carl Hobbsab48004 at 1/300 staining human jejunum tissue sections by IHC-P. A heat mediated antigen retrieval step in citric acid buffer was performed and the tissue was blocked in BSA before incubation with the antibody for 2 hours. A biotinylated rabbit anti-goat antibody was used as the secondary. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab48004)This image is courtesy of an Abreview submitted by Mr Carl Hobbsab48004 diluted 1/200 with TBS/BSA/Azide staining rat d15 embryonic liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat-mediated antigen retrieval step in citric acid buffer at pH6 was performed prior to blocking with 1% BSA for 10 minutes at room temperature. Incubation time with the primary antibody was 16 minutes. A biotinylated rabbit anti-goat was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab48004)Paraffin embedded human placenta tissue stained for Iba1 with ab48004 (2.5 µg/ml) in immunohistochemical analysis. A heat mediated antigen retrieval step in citric acid buffer (pH 6) was performed.
References
This product has been referenced in:
- Wen J et al. WWL70 protects against chronic constriction injury-induced neuropathic pain in mice by cannabinoid receptor-independent mechanisms. J Neuroinflammation 15:9 (2018). Mouse . Read more (PubMed: 29310667) »
- Kim MJ et al. The Anti-Inflammatory Effects of Oral-Formulated Tacrolimus in Mice with Experimental Autoimmune Encephalomyelitis. J Korean Med Sci 32:1502-1507 (2017). Read more (PubMed: 28776347) »
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