Anti-Iba1 antibody (ab48004)

Overview

  • Product nameAnti-Iba1 antibodySee all Iba1 primary antibodies ...
  • Description
    Goat polyclonal to Iba1
  • SpecificityThis antibody is expected to recognise isoform 3 (NP_001614.3) only. This antibody gave excellent staining in paraffin embedded human adult tissue and embyronic rat tissue.
  • Tested applicationsWB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Dog, Pig, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    C-NKQFLDDPKYSSDED

    , corresponding to internal sequence amino acids 28-42 of Human Iba1 (NP_001614.3)

  • Positive control
    • Human Peripheral Blood Mononucleocyte lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab48004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for IHC-FoFr.
  • Target

    • FunctionActin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
    • Tissue specificityDetected in T-lymphocytes and peripheral blood mononuclear cells.
    • Sequence similaritiesContains 2 EF-hand domains.
    • Post-translational
      modifications
      Phosphorylated on serine residues.
    • Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIF 1 antibody
      • AIF-1 antibody
      • AIF1 antibody
      • AIF1 protein antibody
      • AIF1_HUMAN antibody
      • allograft inflammatory factor 1 antibody
      • Allograft inflammatory factor 1 splice variant G antibody
      • allograft inflammatory factor-1 splice variant Hara-1 antibody
      • balloon angioplasty responsive transcription antibody
      • BART 1 antibody
      • G1 antibody
      • G1 putative splice variant of allograft inflamatory factor 1 antibody
      • IBA 1 antibody
      • IBA1 antibody
      • interferon gamma responsive transcript antibody
      • Interferon responsive transcript 1 antibody
      • interferon responsive transcript factor 1 antibody
      • Ionized calcium binding adapter molecule 1 antibody
      • Ionized calcium-binding adapter molecule 1 antibody
      • ionized calcium-binding adapter molecule antibody
      • IRT 1 antibody
      • IRT1 antibody
      • Microglia response factor antibody
      • MRF1 antibody
      • Protein G1 antibody
      see all

    Anti-Iba1 antibody images

    •  ab48004 (2µg/ml) staining of paraffin embedded Mouse Brain shows staining of astrocytes. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

    • Anti-Iba1 antibody (ab48004) at 0.5 µg/ml + Human frontal cortex lysate at 35 µg
      developed using the ECL technique

      Predicted band size : 17 kDa

      Primary incubation was 1 hour

    • All lanes : Anti-Iba1 antibody (ab48004) at 1 µg/ml

      Lane 1 : Human Peripheral Blood Mononucleocyte lysate in RIPA buffer
      Lane 2 : Human Peripheral Blood Mononucleocyte lysate in RIPA buffer with blocking peptide

      Lysates/proteins at 35 µg per lane.

      developed using the ECL technique

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
      Additional bands at : 110 kDa,35 kDa. We are unsure as to the identity of these extra bands.
      1 hour primary antibody incubation. Additional bands of unknown identity observed at 35kDa and 110kDa. These bands were successfully blocked by incubation with the immunising peptide
    • ab48004 at 1/300 staining human jejunum tissue sections by IHC-P. A heat mediated antigen retrieval step in citric acid buffer was performed and the tissue was blocked in BSA before incubation with the antibody for 2 hours. A biotinylated rabbit anti-goat antibody was used as the secondary.

      See Abreview

    • ab48004 diluted 1/200 with TBS/BSA/Azide staining rat d15 embryonic liver tissue sections by IHC-P.  The tissue was formaldehyde fixed and a heat-mediated antigen retrieval step in citric acid buffer at pH6 was performed prior to blocking with 1% BSA for 10 minutes at room temperature.  Incubation time with the primary antibody was 16 minutes.  A biotinylated rabbit anti-goat was used as the secondary

      See Abreview

    • ab48004 at 2.5ug/ml staining human placenta tissue sections by IHC-P. A heat mediated antigen retrieval step in citric acid buffer (pH 6) was performed.

    References for Anti-Iba1 antibody (ab48004)

    ab48004 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry regarding ab48004 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

    Though you have kindly provided some det...

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    Thank you for your reply.

    Your protocol seems comprehensive, although I would strongly recommend including the antigen retrieval step since this has worked well for other customers. We would recommend citrate buffer pH 6, which we do sell as ...

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    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    Wou...

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    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization Yes - 0.1% TritonX-100
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 13 2012

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this case for you and this is also ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rabbit Tissue sections (brain)
    Specification brain
    Fixative Paraformaldehyde
    Permeabilization Yes - triton (20 ul in 10 ml PBS)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Praveen Ballabh

    Verified customer

    Submitted Jul 22 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Dog Tissue sections (spinal cord)
    Specification spinal cord
    Fixative formalin
    Permeabilization Yes - 0.3% PBS-TX
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Joanne Steinauer

    Verified customer

    Submitted Jun 08 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (brain)
    Specification brain
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5%TritonX-100/PBS
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 15 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 07 2009

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (adult liver)
    Specification adult liver
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 17 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"