Synthetic peptide corresponding to Human Iba1 aa 28-42 (internal sequence) (Cysteine residue). NP_001614.3
Sequence:
C-NKQFLDDPKYSSDED
Our Abpromise guarantee covers the use of ab48004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). We recommend blocking in 3-5% milk, not BSA. Blocking in BSA gives high background in WB. |
|
IHC-Fr | Use at an assay dependent concentration. | |
IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Paraffin embedded mouse brain tissue stained for Iba1 with ab48004 (2 µg/ml) in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Primary incubation was 1 hour
Paraformaldehyde fixed mouse brain tissue stained for Iba1 with ab48004 (1/200 dilution) in immunohistochemical analysis. Secondary: Donkey Anti-Goat IgG Cy3® (1/500 dilution).
ab48004 diluted 1/200 with TBS/BSA/Azide staining rat d15 embryonic liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat-mediated antigen retrieval step in citric acid buffer at pH6 was performed prior to blocking with 1% BSA for 10 minutes at room temperature. Incubation time with the primary antibody was 16 minutes. A biotinylated rabbit anti-goat was used as the secondary.
Paraffin embedded human placenta tissue stained for Iba1 with ab48004 (2.5 µg/ml) in immunohistochemical analysis. A heat mediated antigen retrieval step in citric acid buffer (pH 6) was performed.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"