- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameAnti-Iba1 antibody
See all Iba1 primary antibodies - DescriptionGoat polyclonal to Iba1
- SpecificityThis antibody is expected to recognise isoform 3 (NP_001614.3) only. This antibody gave excellent staining in paraffin embedded human adult tissue and embyronic rat tissue. For WB, we recommend blocking in 3-5% milk, NOT BSA. Blocking in BSA gives high background.
- Tested applicationsSuitable for:
WB, IHC-Fr, IHC-Pmore details
Unsuitable for: IHC-FoFr - Species reactivityReacts with: Mouse, Rabbit, Human
Predicted to work with: Cow, Pig, Macaque monkey
- Immunogen
Synthetic peptide corresponding to Human Iba1 aa 28-42 (internal sequence) (Cysteine residue). NP_001614.3
Sequence:C-NKQFLDDPKYSSDED
Database link: P55008 - Positive control
- WB: Human peripheral blood mononucleocyte and frontal cortex lysate. IHC-P: Mouse brain; Human placenta and jejunum; rat embryonic liver tissue.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab48004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). We recommend blocking in 3-5% milk, not BSA. Blocking in BSA gives high background in WB. |
|
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
- FunctionActin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
- Tissue specificityDetected in T-lymphocytes and peripheral blood mononuclear cells.
- Sequence similaritiesContains 2 EF-hand domains.
- Post-translational
modificationsPhosphorylated on serine residues. - Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
- Information by UniProt
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Database links
- Entrez Gene: 280989 Cow
- Entrez Gene: 199 Human
- Entrez Gene: 11629 Mouse
- Entrez Gene: 397271 Pig
- Omim: 601833 Human
- SwissProt: Q9BDK2 Cow
- SwissProt: P55008 Human
- SwissProt: O70200 Mouse
see all -
Alternative names
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
see all
Anti-Iba1 antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab48004)Paraffin embedded mouse brain tissue stained for Iba1 with ab48004 (2 µg/ml) in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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Western blot - Anti-Iba1 antibody (ab48004)Anti-Iba1 antibody (ab48004) at 0.5 µg/ml + human frontal cortex lysate in RIPA buffer at 35 µg
Developed using the ECL technique
Predicted band size : 17 kDaPrimary incubation was 1 hour
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Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody (ab48004)Paraformaldehyde fixed mouse brain tissue stained for Iba1 with ab48004 (1/200 dilution) in immunohistochemical analysis. Secondary: Donkey Anti-Goat IgG Cy3® (1/500 dilution).
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Western blot - Iba1 antibody (ab48004)All lanes : Anti-Iba1 antibody (ab48004) at 1 µg/ml
Lane 1 : Human peripheral blood mononucleocyte lysate in RIPA buffer
Lane 2 : Human peripheral blood mononucleocyte lysate in RIPA buffer with blocking peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique
Predicted band size : 17 kDa
Observed band size : 17 kDa
Additional bands at : 110 kDa,35 kDa. We are unsure as to the identity of these extra bands.1 hour primary antibody incubation. Additional bands of unknown identity observed at 35kDa and 110kDa. These bands were successfully blocked by incubation with the immunising peptide -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iba1 antibody (ab48004)This image is courtesy of an Abreview submitted by Mr Carl Hobbsab48004 at 1/300 staining human jejunum tissue sections by IHC-P. A heat mediated antigen retrieval step in citric acid buffer was performed and the tissue was blocked in BSA before incubation with the antibody for 2 hours. A biotinylated rabbit anti-goat antibody was used as the secondary. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iba1 antibody (ab48004)This image is courtesy of an Abreview submitted by Mr Carl Hobbsab48004 diluted 1/200 with TBS/BSA/Azide staining rat d15 embryonic liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat-mediated antigen retrieval step in citric acid buffer at pH6 was performed prior to blocking with 1% BSA for 10 minutes at room temperature. Incubation time with the primary antibody was 16 minutes. A biotinylated rabbit anti-goat was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iba1 antibody (ab48004)Paraffin embedded human placenta tissue stained for Iba1 with ab48004 (2.5 µg/ml) in immunohistochemical analysis. A heat mediated antigen retrieval step in citric acid buffer (pH 6) was performed.
References for Anti-Iba1 antibody (ab48004)
This product has been referenced in:
- Hurtado-Alvarado G et al. A2A Adenosine Receptor Antagonism Reverts the Blood-Brain Barrier Dysfunction Induced by Sleep Restriction. PLoS One 11:e0167236 (2016). WB, IHC ; Rat . Read more (PubMed: 27893847) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"