![Western blot - Id1 antibody [2456C1a] (ab66495) image](#ab66495_1.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-Id1 antibody [2456C1a]
See all Id1 products (2) ...
Description
Mouse monoclonal [2456C1a] to Id1
Tested applications
IHC-P, WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment from the internal region of human Id1
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (~95%) fetal bovine serum.
Clonality
Monoclonal
Clone number
2456C1a
Isotype
IgG1
Research areas
Cancer >> Invasion/microenvironment >> Angiogenesis >> Other
Cancer >> Signal transduction >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Western blot - Id1 antibody [2456C1a] (ab66495)
![Western blot - Id1 antibody [2456C1a] (ab66495) image](/ps/datasheet/Images/66/ab66495/ab66495_1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Dot Blot
Application notes
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ID proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID proteins contribute to cell growth, senescence, differentiation and angiogenesis. Id1 mRNA is highly expressed in heart, lung and kidney and has lower expression in brain and liver. Two transcript variants encoding different isoforms have been found for this gene.
Cellular localization
Nuclear
Alternative names
- DNA binding protein inhibitor ID 1 antibody
- DNA binding protein inhibitor ID1 antibody
- Dominant negative helix loop helix protein antibody
see all
Database links
- Entrez Gene: 3397 Human
- Omim: 600349 Human
- SwissProt: P41134 Human
- Unigene: 504609 Human
Anti-Id1 antibody [2456C1a] images:
Western blot - Id1 antibody [2456C1a] (ab66495)
Predicted band size : 16 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Western blot analysis of immunized recombinant protein, using ab66495.
References for Anti-Id1 antibody [2456C1a] (ab66495)
ab66495 has not yet been referenced specifically in any publications.
Publishing research using ab66495? Please let us know so that we can cite the reference in this datasheet
![Id1 antibody [2456C1a] for IHC-P in Human (66495)](/ps/Reviews/Images/ab13609_81500.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"