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Western blot - Id1 antibody (ab52998)
(enlarge)
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Product Name
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Id1 antibody
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See all Id1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Id1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus Id1.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 16 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
HepG2 Whole Cell, Mouse Pancreas Tissue, Mouse Liver Tissue.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Invasion/microenvironment >> Angiogenesis >> Other
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> CD markers
Cancer >> Signal transduction >> Other
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Immunology >> Cell Type Markers >> CD >> Adhesion
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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ID proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID proteins contribute to cell growth, senescence, differentiation and angiogenesis. Id1 mRNA is highly expressed in heart, lung and kidney and has lower expression in brain and liver. Two transcript variants encoding different isoforms have been found for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.400 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Id1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Id1 antibody images, references, products related to ab52998 and other tools.
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Id1 antibody images:
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 Western blot - Id1 antibody (ab52998)
All lanes : Id1 antibody (ab52998) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
The 22 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Id1.
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Search PubMed (MEDLINE) for references to Id1
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Id1 antibody - more information
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Customer reviews (feedback) regarding Id1 antibody |
Customer FAQs regarding Id1 antibody |
Protocols for Id1 antibody |
Price and availability of products related to Id1 antibody |
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Id1 antibody - related products:
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Id1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Id1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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