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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-Id1 antibody
See all Id1 products (2) ...
Description
Rabbit polyclonal to Id1
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus Id1.
(Peptide available as ab86654.)
Positive control
This antibody gave a positive signal in the following lysates: HepG2 Whole Cell, Mouse Pancreas Tissue, Mouse Liver Tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Angiogenesis >> Other
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cancer >> Tumor biomarkers >> Other
Cancer >> Tumor immunology >> CD markers
Cancer >> Signal transduction >> Other
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Immunology >> Cell Type Markers >> CD >> Adhesion
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Western blot - Id1 antibody (ab52998)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 22 kDa (predicted molecular weight: 16 kDa).
Target
Relevance
ID proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID proteins contribute to cell growth, senescence, differentiation and angiogenesis. Id1 mRNA is highly expressed in heart, lung and kidney and has lower expression in brain and liver. Two transcript variants encoding different isoforms have been found for this gene.
Cellular localization
Nuclear
Alternative names
- DNA binding protein inhibitor ID 1 antibody
- DNA binding protein inhibitor ID1 antibody
- Dominant negative helix loop helix protein antibody
see all
Database links
- Entrez Gene: 497011 Cow
- Entrez Gene: 3397 Human
- Entrez Gene: 15901 Mouse
- Entrez Gene: 25261 Rat
- Omim: 600349 Human
- SwissProt: P41134 Human
- SwissProt: P20067 Mouse
- SwissProt: P41135 Rat
see all
Anti-Id1 antibody images:
Western blot - Id1 antibody (ab52998)
All lanes : Anti-Id1 antibody (ab52998) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
The 22 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Id1.
References for Anti-Id1 antibody (ab52998)
ab52998 has not yet been referenced specifically in any publications.
Publishing research using ab52998? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"