Anti-IgE antibody [4C3] (ab106494)
Key features and details
- Mouse monoclonal [4C3] to IgE
- Suitable for: Sandwich ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-IgE antibody [4C3]
See all IgE primary antibodies -
Description
Mouse monoclonal [4C3] to IgE -
Host species
Mouse -
Specificity
ab106494 is specific for Human IgE. It reacts with IgE purified from blood but does not cross-react with Human anti-IgG1, IgG2, IgG3, IgG4, IgA and IgM
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Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse -
Immunogen
Fusion protein corresponding to Human IgE.
Database link: P01854 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4C3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use a concentration of 5 µg/ml.
for coating plates. Use ab106493 for detection. |
Notes |
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Sandwich ELISA
Use a concentration of 5 µg/ml. for coating plates. Use ab106493 for detection. |
Target
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Relevance
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 3497 Human
- Omim: 147180 Human
- SwissProt: P01854 Human
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Alternative names
- Igh2 antibody
- IGHE antibody
- IGHEP1 antibody
- Immunoglobulin heavy constant epsilon antibody
Images
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ab106494 used at 5 µg/ml for coating ELISA plates to capture Human IgE. Detection utilised ab106493 at 1/1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab106494 has been referenced in 2 publications.
- Zhang Y et al. An allergenic plant calmodulin from Artemisia pollen primes human DCs leads to Th2 polarization. Front Immunol 13:996427 (2022). PubMed: 36248805
- Leung S et al. Multiplexed immunoassay approach to characterize antidrug antibody like specific reactivity. Bioanalysis 11:703-712 (2019). PubMed: 30994012