Overview
- Product nameIgG Human ELISA KitSee all IgG kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeSandwich
- Sensitivity< 150 pg/ml
- Range0.021 ng/ml - 15 ng/ml
- Recovery
117 %
Sample specific recovery Sample type Average % Range Serum 116.1 105% - 123% Plasma 118.1 99% - 126% - Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Human IgG ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IgG in serum and plasma.
This assay employs an antibody specific for Human IgG coated on a 96-well plate. Standards and samples are pipetted into the wells and IgG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Notes
The Human immune system consists of two functional components classified as the innate system (the physical, biochemical and cellular barriers), and the adaptive immune system (including lymphocytes and immunoglobulins). Immunoglobulins are the key elements of the humoral immune response in vertebrate against parasitic invasion.
The polypeptide chains of immunoglobulins composed of two identical heavy (H) chains and two identical light (L) chains linked together by inter-chain disulfide bonds. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membranes. Based upon the variation of the constant region of the heavy chain, nine immunoglobulin heavy chain isotypes are found in humans: IgA (with subclasses IgA1 and IgA2), IgD, IgE, IgM, and IgG (with subclasses IgG1, IgG2, IgG3, and IgG4).
IgG is the predominant immunoglobulin in the serum, which accounts for 75% of the total serum antibody of healthy individuals. IgG has a molecular weight of about 150 kDa. Four distinct subgroups of human IgG (IgG1, IgG2, IgG3, and IgG4) were first demonstrated in the 1960’s by using polyclonal antisera prepared in animals immunized with human myeloma proteins.
The capture antibody of the kit ab100547 detects the Fc portion of the heavy chain of all subclasses of human IgG and the detection antibody detects the F(ab’)2 fragment of IgG.
- Tested applicationsSandwich ELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 4,000X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 2 x 15ml Biotinylated anti-Human IgG 2 vials IgG Microplate (12 x 8 wells) 1 unit Recombinant Human IgG Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml - Research Areas
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Alternative names
- Ig gammaIg gamma-1 chain C regionIGHG1Immunoglobulin G
- Entrez Gene: 3500 Human
- Omim: 147100 Human
- Omim: 147110 Human
- SwissProt: P01857 Human
- SwissProt: P01859 Human
Applications
Our Abpromise guarantee covers the use of ab100547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
IgG Human ELISA Kit images
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Typical standard curveRepresentative standard curve using ab100547
Protocols
References for IgG Human ELISA Kit (ab100547)
ab100547 has not yet been referenced specifically in any publications.
