IgG1 Human ELISA Kit (ab100548)
- Product nameIgG1 Human ELISA KitSee all IgG1 kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity< 0.05 ng/ml
- Range0.05 ng/ml - 40 ng/ml
Sample specific recovery Sample type Average % Range Serum 87 75% - 97% Plasma 81.5 71% - 94%
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s IgG1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IgG1 in serum and plasma.
This assay employs an antibody specific for Human IgG1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IgG1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IgG1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 2,000X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 2 x 15ml Biotinylated anti-Human IgG1 2 vials IgG1 Microplate (12 x 8 wells) 1 unit Recombinant Human IgG1 Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- Research Areas
- RelevanceThere are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
- Ig gamma 1 chain C region
Our Abpromise guarantee covers the use of ab100548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for IgG1 Human ELISA Kit (ab100548)
ab100548 has not yet been referenced specifically in any publications.