Anti-IgG3Ch antibody (ab43683)
- Product nameAnti-IgG3Ch antibodySee all Human IgG3 primary antibodies ...
- DescriptionMouse polyclonal to Human IgG3
- SpecificityThis antibody reacts with the IgG3 heavy chain constant region.
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Vector coding for a partial recombinant fusion protein, corresponding to amino acids 1-100 of Human IgG3. Target Sequence used to make the antibody: ASTKGPSVFP LAPCSRSTSG GTAALGCLVK DYFPEPVTVS WNSGALTSGV HTFPAVLQSS GLYSLSSVVT VPSSSLGTQT YTCNVNHKPS NTKVDKRVEL. Full length sequence: Accession Number: P01860, 377 amino acids.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 50% Glycerol, Whole serum
- PurityWhole antiserum
- Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab43683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- RelevanceIgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
- Cellular localizationSecreted
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Anti-IgG3Ch antibody images
All lanes : Anti-IgG3Ch antibody (ab43683) at 1/1000 dilution
Lane 1 : 20µg of a total protein extract from E coli with ~50ng to 500ng of a fusion protein of an irrelevant antigen (negative control lane).
Lane 2 : 20µg of a total protein extract from E coli with ~50ng to 500ng of the antigen (fusion protein).
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 57 kDa
References for Anti-IgG3Ch antibody (ab43683)
ab43683 has not yet been referenced specifically in any publications.