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Anti-Ihh antibody [EP1192Y] (ab52919)

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Overview

Product name

Anti-Ihh antibody [EP1192Y]
See all Ihh products (5) ...

Description

Rabbit monoclonal [EP1192Y] to Ihh

Tested applications

WB, IP, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues near the N-terminus of human IHH.

Positive control

Human fetal colon tissue, fetal brain lysate.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1192Y

Isotype

IgG

Research areas

Stem Cells >> Signaling Pathways >> Hedgehog >> Secreted
Stem Cells >> Signaling Pathways >> Hedgehog >> Surface Molecules

  • Western blot - Ihh antibody [EP1192Y] (ab52919)Western blot - Ihh antibody [EP1192Y] (ab52919) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Ihh antibody [EP1192Y] (ab52919)Immunohistochemistry (Paraffin-embedded sections) - Ihh antibody [EP1192Y] (ab52919) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for or Flow Cyt.

Target

Relevance

Ihh is an intercellular signalling peptide essential for a variety of patterning events during development. It binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Ihh induces the expression of parathyroid hormone-related protein (PTHRP) and is implicated in endochondral ossification, possibly regulating the balance between growth and ossification of the developing bones.

Cellular localization

Cytoplasmic and Secreted

Alternative names

  • HHG2 antibody
  • Indian Hedgehog antibody

Database links

see all

Anti-Ihh antibody [EP1192Y] images:

  Western blot - Ihh antibody [EP1192Y] (ab52919)

Western blot - Ihh antibody [EP1192Y] (ab52919)

Anti-Ihh antibody [EP1192Y] (ab52919) at 1/10000 dilution + Human fetal brain lysate at 10 µg

Secondary
Goat anti rabbit hrp conjugated at 1/2000 dilution

Predicted band size : 45 kDa
Observed band size : 45 kDa

  Immunohistochemistry (Paraffin-embedded sections) - Ihh antibody [EP1192Y] (ab52919)

Immunohistochemistry (Paraffin-embedded sections) - Ihh antibody [EP1192Y] (ab52919)

Ab52919 at 1/250 dilution staining human fetal colon tissue; paraffin embedded.

References for Anti-Ihh antibody [EP1192Y] (ab52919)

This product has been referenced in:

  • Rehm Aet al. Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs. Blood 118:1020-33 (2011). IHC-Fr; Mouse.Read more (PubMed: 21586747) »

See 1 publication for this product

Publishing research using ab52919? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"