Anti-Influenza A Virus Hemagglutinin H1 antibody [1.B.408] (ab61986)

Thanks for your kindly reply, the information of this product as follow:



Name: one vail of ab61986 (Anti-Influenza A Virus Hemagglutinin H1 antibody [1.B.408])

Lot: 864175

Po: 1067432

Order date: 2012/4/12



Please check it and help her to create a credit note

Thank you very much!

Best regards

ANSWER:

Thank you for contacting us.

Your credit note ID is 20852.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Thanks for your kindly reply, after contact this customer, she would like to have a credit note,


could you please help her to create a credit note ?

I deeply appreciate your great assistance!



Best regards

ANSWER:

Thank you for your enquiry.

My colleague is out of office this week and she has asked me to look after her customers.

Could you please confirm the Order details and how many vials are on the order.

I look forward to hearing from you soon.

Thanks for your kindly reply, this customer indicated the amount of this protein her used was approximately 0.24ug, and the antibody stored at -20C then protein stored at +4C, furthermore she also indicated this repair of these products was suggested by ####, the original letter as follow:


Dear Technical Service

Thank you for contacting us.

Product ab61986 works as an inhibitor in Hemaglutinnin Assay, it is a suitable option for your assay:

http://www.abcam.com/influenza-a-virus-hemagglutinin-h1-antibody-1-b-408-ab61986.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

ANSWER:

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments. As the antibody does not seem to be neutralizing the protein in the hemolytic assays, I would be pleased to arrange acredit noteor free of charge replacement for the ab61986Influenza A Virus Hemagglutinin H1 antibody [1.B.408].

I look forward to hearing from you with details of how you would like to proceed.

Dear Abcam technical support team:

This customer has already purchased ab61986 (Anti-Influenza A Virus Hemagglutinin H1 antibody [1.B.408]), ab69741 (Influenza A Virus Hemagglutinin H1 protein) and conducted “Hemagglutinin Inhibition Assay” with human RBC sample, however, the result shows no significant different with the negative control, therefore, she offered her experiment step and data in this letter, could please check it and offer any suggestions to help her.


The product informations as follow:

ab61986 (Anti-Influenza A Virus Hemagglutinin H1 antibody [1.B.408])

Lot: 864175

Po: 1067432



ab69741 (Influenza A Virus Hemagglutinin H1 protein)

Lot: gr62763-3

Po: 1051535



Thanks for your kindly help

Best regards

ANSWER:

Thank you for your reply and for kindly providing the information again.

This will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you thatthese antibodies ab61986and ab69741 are tested and covered by our 6 month guarantee for use ininhibition assays. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing the details I would appreciate ifit is possible toprovide full further information on the recombinant protein used. This will help my understanding of the assay and how it is working with these antibodies.

1. Was the recombinant protein full length influenza A virus Hemagglutinin H1 protein? Does it contain the immunogen sequence for these antibodies?

2. How was it prepared?

3. How much HA recombinant protein(in ug) was added to each well? Has this been optimised with the dilution of antibody? It may be there is too much HA recombinant protein for the amount of antibody to totally neutralizse.

3. Do you have the supplier and product code?

4. Do you get the same results from both antibodies?

Many thanks for your continued cooperation. I look forward to hearing from you with the requested details.

Here is an answer from our customer:

I will be happy to present all necessary information for the troubleshooting process.

"It looks like ab66189 and ab61986 are being use as detection antibodies which are in turn detected by anti-mouse secondary antibodies? Is this the case?"

Yes, we used these antibodies as detection antibodies which are in turn detected by anti-mouse secondary antibodies (biotin- labeled secondary antibody, Sigma)

"If so I was wondering how much of these antibodies are being added to the wells (eg. 1ug/well) and if any other dilutions have been tried?"

We added to the wells only 1.01ug/well of ab66189 and 0.64ug/well of ab61986.

Hope that will be helpful.

ANSWER:

Thanks for the additional information.

It does appear as if these two antibodies are not working as expected in ELISA. I would be happy to replace them for you with any other primary antibodies.

I will just need the order reference number or PO number associated with the original order to process your request.