Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189)

Thanks for your answer. Our customer would like to replace ab66189 for: Anti-Influenza A Virus antibody (Biotin) (ab119981) Please add this antibody to our back order xxxx. Antibody ab66189 was bought at ORDER #xxxx

ANSWER:

Thank you for contacting Abcam.

The free of charge replacement of ab66189 with ab119981 has been placed on order xxxx as you have requested.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Here is an answer from our customer:

I will be happy to present all necessary information for the troubleshooting process.

"It looks like ab66189 and ab61986 are being use as detection antibodies which are in turn detected by anti-mouse secondary antibodies? Is this the case?"

Yes, we used these antibodies as detection antibodies which are in turn detected by anti-mouse secondary antibodies (biotin- labeled secondary antibody, Sigma)

"If so I was wondering how much of these antibodies are being added to the wells (eg. 1ug/well) and if any other dilutions have been tried?"

We added to the wells only 1.01ug/well of ab66189 and 0.64ug/well of ab61986.

Hope that will be helpful.

ANSWER:

Thanks for the additional information.

It does appear as if these two antibodies are not working as expected in ELISA. I would be happy to replace them for you with any other primary antibodies.

I will just need the order reference number or PO number associated with the original order to process your request.

Please find answers of customer in attached file.

ANSWER:

Thanks so much for the additional information.

It looks like ab66189 and ab61986 are being use as detection antibodies which are in turn detected by anti-mouse secondary antibodies? Is this the case?

If so I was wondering how much of these antibodies is being added to the wells (eg. 1ug/well) and if any other dilutions have been tried?

Thanks again for your patience with the troubleshooting process. Please know that if its determined there is an issue with these two antibodies I am happy to offer replacements, credits or refunds.

One of customers is not happy with antibodies that he bought second time. Last time they were bought last year and did not work either.

I have enclosed questionnaire below my signature.

ANSWER:

Thank you for your enquiry and for completing the questionnaire.

I just have a few follow up questions:

1. Please describe the sample loaded onto well?

2. Did other antibodies work in ELISA assay with this same sample?

3. Please describe howab66189 didnot work? No signal/high background/non specific?

Thanks in advance for the additonal information.

I'm looking for an H1N1 antibody that cross-reacts with Mexico/2009 strain.

ANSWER:

I would still need the accession number for your strain, but I just wanted to let you know of additional information about 2 of our products.


Concerning ab61986, this antibody recognizes all Hemagglutinin from H1N1 strains. The immunogen used to produce this antibody is from Influenza A/USSR/90/77. The epitope is in the range aa206-225 (based on accesssion # ABD95350).



For ab66189, this antibody has not been tested for reactivity to Influenza A/Mexico/2009 strain. The immunogen used to develop ab66189was purified Influenza A/New Caledonia/20/99 (H1N1) (not a synthetic peptide sequence). This clone has been tested to show reactivity to hemagglutinin H1, but the exact epitope location on H1 has not been mapped. For this reason, we are not able
to do a BLAST to compare to A/Mexico/2009.



If you can provide the sequence, Icanget the BLAST done on ab91531.



I look forward to your reply.