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Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189)

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Overview

Product name

Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M]
See all Influenza A Virus Hemagglutinin H1 products (3) ...

Description

Mouse monoclonal [B219M] to Influenza A Virus Hemagglutinin H1

Specificity

Reacts with Hemagglutinin from Influenza A Virus of H1 serotype. ab66189 has tested positive against the following Influenza A strains: A/Beijing/262/95 (H1N1) A/New Caledonia/20/99 (H1N1) A/Taiwan/1/86 (H1N1) ab66189 has tested negative against the following Influenza A strains: A/Japan/305/57 (H2N2) A/Panama/2007/99 (H3N2) A/Shangdong/9/93 (H3N2) A/Netherlands/219/03 (H7N7) A/New York/107/2003 (H7N2)

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with Influenza A Virus.

Immunogen

Purified Influenza A/NewCaledonia/20/99 virus (strain H1N1)

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Purification notes

>90% pure (SDS-PAGE).

Clonality

Monoclonal

Clone number

B219M

Myeloma

Sp2/0

Isotype

IgG1

Research areas

Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza

  • Western blot - Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189)Western blot - Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab66189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Influenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells.

Alternative names

  • HA antibody
  • Hemagglutinin antibody
  • Hemagglutinin HA antibody

Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] images:

  Western blot - Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189)

Western blot - Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189)

Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189) at 5 µg/ml + Influenza A/Taiwan/1/86 at 0.5 µg

Secondary
HRP-conjugated anti-mouse IgG

Performed under reducing conditions.

Protocol notes:

  • SDS-PAGE in reducing conditions, 10 % acrylamide
  • After SDS-PAGE transfer to nitrocellulose membrane
  • Blocking in PBS, containing 0.5% Tween 20, 10% dry milk (w/v) – incubation 10 minutes at room temperature.
  • Primary antibody: 5 µg/ml diluted in PBS containing 0.5% Tween 20 – incubation 1 hour at room temperature.
  • Secondary antibody: HRP-conjugated anti-mouse IgG diluted in PBS, containing 0.5% Tween 20 – incubation 1hour at room temperature.
  • Diaminobenzidine 50 µg/ml – 20 min

References for Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189)

This product has been referenced in:

  • Farahmand Bet al. Influenza Virus Hemagglutinin: A Model for Protein N-Glycosylation in Recombinant Escherichia coli. Intervirology : (2011). WB.Read more (PubMed: 21734350) »

See 1 publication for this product

Publishing research using ab66189? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"