Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189)

Overview

  • Product nameAnti-Influenza A Virus Hemagglutinin H1 antibody [B219M]See all Influenza A Virus Hemagglutinin H1 primary antibodies ...
  • Description
    Mouse monoclonal [B219M] to Influenza A Virus Hemagglutinin H1
  • SpecificityReacts with Hemagglutinin from Influenza A Virus of H1 serotype. ab66189 has tested positive against the following Influenza A strains: A/Beijing/262/95 (H1N1) A/New Caledonia/20/99 (H1N1) A/Taiwan/1/86 (H1N1) ab66189 has tested negative against the following Influenza A strains: A/Japan/305/57 (H2N2) A/Panama/2007/99 (H3N2) A/Shangdong/9/93 (H3N2) A/Netherlands/219/03 (H7N7) A/New York/107/2003 (H7N2)
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with Influenza A Virus.
  • Immunogen

    Purified Influenza A/NewCaledonia/20/99 virus (strain H1N1)

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notes>90% pure (SDS-PAGE).
  • Clonality Monoclonal
  • Clone numberB219M
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab66189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 5 µg/ml.

Target

  • RelevanceInfluenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells.
  • Alternative names
    • HA antibody
    • Hemagglutinin antibody
    • Hemagglutinin HA antibody

Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] images

  • Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189) at 5 µg/ml + Influenza A/Taiwan/1/86 at 0.5 µg

    Secondary
    HRP-conjugated anti-mouse IgG

    Performed under reducing conditions.

References for Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189)

This product has been referenced in:
  • Price JV  et al. Characterization of influenza vaccine immunogenicity using influenza antigen microarrays. PLoS One 8:e64555 (2013). Read more (PubMed: 23734205) »
  • Farahmand B  et al. Influenza Virus Hemagglutinin: A Model for Protein N-Glycosylation in Recombinant Escherichia coli. Intervirology : (2011). WB . Read more (PubMed: 21734350) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam. The free of charge replacement of ab66189 with ab119981 has been placed on order xxxx as you have requested. I hope that this information is helpful. Please let me know if you have any questions or there are ot...

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Thanks for the additional information.

It does appear as if these two antibodies are not working as expected in ELISA. I would be happy to replace them for you with any other primary antibodies.

I will just need the order reference ...

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Thanks so much for the additional information.

It looks like ab66189 and ab61986 are being use as detection antibodies which are in turn detected by anti-mouse secondary antibodies? Is this the case?

If so I was wondering how much o...

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Thank you for your enquiry and for completing the questionnaire.

I just have a few follow up questions:

1. Please describe the sample loaded onto well?

2. Did other antibodies work in ELISA assay with this same sample? Read More

I would still need the accession number for your strain, but I just wanted to let you know of additional information about 2 of our products.


Concerning ab61986, this antibody recognizes all Hemagglutinin from H1N1 strains. The immunoge...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"