You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Influenza A Virus Hemagglutinin H1 protein (ab69741)

Submit an Abreview Q&A (9)Specific References (1)

Overview

  • Product nameInfluenza A Virus Hemagglutinin H1 protein
  • Protein descriptionRecombinant Full Length Influenza A Virus Hemagglutinin H1 produced using insect cells infected with A9440.1a recombinant baculovirus expressing recombinant H1N1 A/New Caledonia/20/99, 57,500 dalton. Swiss ID = DQ508857.
  • Expression hostBaculovirus infected insect cells

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesPurity is greater than 90.0% as determined by SDS-PAGE, HA1 and HA2 bands are observed using SDS-PAGE under reducing conditions.
  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: 0.005% Tween 20, 150mM Sodium chloride, 10mM Sodium phosphate, pH 7.2
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab69741 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use a concentration of 0.1 - 1 µg/ml.
    ELISA ELISA: Use at an assay dependent concentration.

    Protein info

    • Alternative names
        HAHemagglutininHemagglutinin HA
  • RelevanceInfluenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells.

    • References for Influenza A Virus Hemagglutinin H1 protein (ab69741)

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab69741 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab69741 Submit a Question for ab69741

    Displaying 1 - 9 of 9 results for Abreviews and Q&A

    10
    Votes: Highest First

    Where are the binding sites located on the antigen? Is there more then one site that reacts with Anti-Influenza A Virus Hemagglutinin H1 Antibody (ab61986)?

    The binding site on the antigen is mostly dependent on the binding properties of the antibody, not of the antigen itself. Ab61986 is a monoclonal antibody raised against the full-length protein. Since it is a monoclonal antibody, it only has one binding s...

    Read More

    May I ask further information for the following Abcam products?


    Experiment background - Antibody is used to direct bind to protein on a gold sensor surface.


    Q1. Is ab82455 http://www.abcam.com/H5-H5N1-antibody-8D2-ab82455...

    Read More

    Thank you for contacting us.

    Q1. Is ab82455 Anti-H5 (H5N1) antibody [8D2] specific bind to ab123220 (Influenza A, Avian, Vietnam 1203/04, H5N1, Hemagglutinin protein)?

    Answer: Yes, the antibody will recognize this protein, ab123220. ...

    Read More

    I have an aliquot of your protein Ha1, cat# ab69741, to use as a positive control on Westerns. I am consistently getting two bands from this purified protein on the gel/Western. Compared to markers, either the bands are at about 60 and 50 kDa or they are ...

    Read More

    Thank you for contacting us.

    The hemagglutinin precursor protein is cleaved into two chains, HA1 and HA2.I can see from the notes on the datasheet that this Influenza A Virus Hemagglutinin H1 protein will give two bands, one for HA1 and one for H...

    Read More

    Thanks for your kindly reply, the information of this product as follow:



    Name: one vail of ab61986 (Anti-Influenza A Virus Hemagglutinin H1 antibody [1.B.408])

    Lot: 864175

    Po: 1067432

    Order date: 201...

    Read More

    Thank you for contacting us.

    Your credit note ID is 20852.

    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in o...

    Read More

    Thanks for your kindly reply, after contact this customer, she would like to have a credit note,


    could you please help her to create a credit note ?

    I deeply appreciate your great assistance!



    Best regards

    Thank you for your enquiry.

    My colleague is out of office this week and she has asked me to look after her customers.

    Could you please confirm the Order details and how many vials are on the order.

    I look forward to hearing f...

    Read More

    Thanks for your kindly reply, this customer indicated the amount of this protein her used was approximately 0.24ug, and the antibody stored at -20C then protein stored at +4C, furthermore she also indicated this repair of these products was suggested by #...

    Read More

    Thank you for your message and for providing this further information.

    I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments. As the antibody does not s...

    Read More

    Dear Abcam technical support team:

    This customer has already purchased ab61986 (Anti-Influenza A Virus Hemagglutinin H1 antibody [1.B.408]), ab69741 (Influenza A Virus Hemagglutinin H1 protein) and conducted “Hemagglutinin Inhibition Assa...

    Read More

    Thank you for your reply and for kindly providing the information again.

    This will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    I would like to reassure you thatthese a...

    Read More


    pouvez-vous me dire si les protéines suivantes sont taggés et me donner leurs poids moléculaires avec tag:
    - ab69741
    - ab69748
    - ab69749
    - ab91276
    - ab67704

    Merci de nous avoir contactés.

    ab67704 : tag GST, 40 kDa
    ab69741 : pas de tag, 57.5 kDa
    ab69748 : pas de tag, 64.5 kDa
    ab69749 : pas de tag, 70 kDa
    ab91276 : pas de tag, 24 kDa

    J'espère que ces informa...

    Read More

    Is this protein in trimeric or monomeric form?

    Thank you for contacting us. Here is the information I received from the originating laboratory: Recombinant Full-Length H1N1 New Caledonia/20/99 is TRIMERIC FORM, glycosylated with N-linked sugars, produced using baculovirus vectors in insect cells and i...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"