Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)

Overview

  • Product nameAnti-Influenza A Virus M2 Protein antibody [14C2]See all Influenza A Virus M2 Protein primary antibodies ...
  • Description
    Mouse monoclonal [14C2] to Influenza A Virus M2 Protein
  • Tested applicationsInhibition Assay, Flow Cyt, ICC/IF, ICC, IP, WBmore details
  • Immunogen

    Tissue, cells or virus corresponding to Influenza A Virus M2 Protein. Full length M2 protein from A/WSN/33-infected CV1 cell lysate.

  • EpitopeDetects the N-terminal of the Influenza A Virus M2 Protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notes Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
  • ClonalityMonoclonal
  • Clone number14C2
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab5416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa.

Target

  • RelevanceThe Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
  • Cellular localizationVirion membrane. Apical cell membrane; Single-pass type III membrane protein.
  • Alternative names
    • Influenza A virus matrix protein M2 antibody
    • M antibody
    • M2 antibody
    • Matrix protein 2 antibody
    • Membrane ion channel M2 antibody
    • Membrane protein M2 antibody
    • Proton channel protein M2 antibody
    see all

Anti-Influenza A Virus M2 Protein antibody [14C2] images

  • Immunofluorescence staining of infected MDCK cells using ab5416.

References for Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)

This product has been referenced in:
  • Ohtsuka J  et al. Vero/BC-F: an efficient packaging cell line stably expressing F protein to generate single round-infectious human parainfluenza virus type 2 vector. Gene Ther N/A:N/A (2014). WB . Read more (PubMed: 24942630) »
  • Wang L  et al. Nanoclusters self-assembled from conformation-stabilized influenza M2e as broadly cross-protective influenza vaccines. Nanomedicine N/A:N/A (2013). Read more (PubMed: 23988715) »

See all 12 Publications for this product

Product Wall

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Finally, I have a price quote and custom made confirmation from laboratory. We are happy to provide this product at lower cost than ab5416. The price would be £194 per vial or £582 for 3 vial...

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I have sent a request to laboratory for quote; I will send you the details soon.

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I just spoken to my colleague in laboratory; they have suggested that in order to justify the production cost for making it BSA and azide free at least 3 vials should be ordered. Could you let me know if you will buy ...

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Thank you for contacting us.

We can provide this product, BSA and azide free as a special request. Could you let me know how many vials you would like to buy?

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Our Influenza A M2 Monoclonal Antibody (14C2) - product #ab5416 was shown to detect influenza A virus M2 strain A/PR/8/34 in the following reference:



Zebedee SL, Lamb RA"Influenza A vi...

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Thank you foryour reply and forkindly providing the requested information.

I have investigated this with our source for the antibody and reviewed the details. It is worth consideringthat the Nandrop will be unable to distinguish between IgG ...

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Thank you for contacting Abcam regarding this,
I have had the chance to speak with the lab about your question. We have not tested whether this antibody can be used as a neutralizing antibody. The authors in the following paper used this antibody i...

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I would suggest to prepare buffers that are recommended in the following Western Blotting protocol : http://www.abcam.com/index.html?pageconfig=resource&rid=13045 .

Thank you very much for your inquiry. I have contacted the laboratory for information on the ab5416. However the laboratory has not tested this antibody neither on your strain. I did a sequence alignement of the immunogen and your sequence. Please see ...

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Thank you for your enquiry. This product was created against whole M2 protein purified from A/WSN/33-infected CV1 cell lysate. Unfortunately, the exact epitope for this polyclonal antibody is not known at this time. The antibody was not tested aga...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"