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Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)

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Overview

Product name

Anti-Influenza A Virus M2 Protein antibody [14C2]
See all Influenza A Virus M2 Protein products (3) ...

Description

Mouse monoclonal [14C2] to Influenza A Virus M2 Protein

Tested applications

Flow Cyt, ICC/IF, IF, ICC, IP, WBmore details

Immunogen

Full length M2 protein from A/WSN/33-infected CV1 cell lysate.

Epitope

Detects the N-terminal of the Influenza A Virus M2 Protein.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA

Concentration

Concentration information loading...

Purity

Protein G purified

Primary antibody notes

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

Clonality

Monoclonal

Clone number

14C2

Isotype

IgG1

Research areas

Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza

  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab5416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Flow Cyt: Use at an assay dependent dilution (PMID 20025741).
ICC: Use at a concentration of 1 µg/ml.
ICC/IF: Use at an assay dependent concentration.
IF: Use at a concentration of 1 µg/ml.
IP: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 15 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.

Cellular localization

Virion membrane. Apical cell membrane; Single-pass type III membrane protein.

Alternative names

  • Influenza A virus matrix protein M2 antibody
  • M antibody
  • M2 antibody
  • Matrix protein 2 antibody
  • Membrane ion channel M2 antibody
  • Membrane protein M2 antibody
  • Proton channel protein M2 antibody
see all

Anti-Influenza A Virus M2 Protein antibody [14C2] images:

  Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)

Immunofluorescence staining of infected MDCK cells using ab5416.

References for Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)

This product has been referenced in:

  • Song JM  et al. Influenza virus-like particles containing m2 induce broadly cross protective immunity. PLoS One 6:e14538 (2011). WB.Read more (PubMed: 21267073) »
  • Wu CY  et al. Mammalian expression of virus-like particles for advanced mimicry of authentic influenza virus. PLoS One 5:e9784 (2010). WB.Read more (PubMed: 20339535) »

See all 9 publications for this product

Publishing research using ab5416? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"