Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)

Overview

• Product nameAnti-Influenza A Virus M2 Protein antibody [14C2]See all Influenza A Virus M2 Protein primary antibodies ...
• Description
  Mouse monoclonal [14C2] to Influenza A Virus M2 Protein
• Tested applicationsFlow Cyt, ICC/IF, ICC, IP, WB more details
• Immunogen

  Full length M2 protein from A/WSN/33-infected CV1 cell lysate.

• EpitopeDetects the N-terminal of the Influenza A Virus M2 Protein.
• General notes

  
  

  Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS, 1mg/ml BSA
• Concentration information loading...
• PurityProtein G purified
• Primary antibody notes Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
• Clonality Monoclonal
• Clone number14C2
• IsotypeIgG1
• Research Areas
  • Microbiology
  • Organism
  • Virus
  • RNA Virus
  • single stranded RNA negative strand virus
  • Influenza

Applications

Target

• RelevanceThe Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
• Cellular localizationVirion membrane. Apical cell membrane; Single-pass type III membrane protein.

• Alternative names
  Influenza A virus matrix protein M2 antibodyM antibodyM2 antibody

  Matrix protein 2 antibodyMembrane ion channel M2 antibodyMembrane protein M2 antibodyProton channel protein M2 antibody

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Anti-Influenza A Virus M2 Protein antibody [14C2] images

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  Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)
  Immunofluorescence staining of infected MDCK cells using ab5416.