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Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC) (ab20921)

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Overview

Product name

Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC)
See all Influenza A Virus Nucleoprotein products (10) ...

Description

Mouse monoclonal [431] to Influenza A Virus Nucleoprotein (FITC)

Conjugation

FITC

Conjugation notes

Conjugated with high purity isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Specificity

Specific for nucleoprotein of all Influenza A strains.

Tested applications

ICC/IF, Flow Cytmore details

Cross reactivity

Reacts with the Influenza A virus.

Immunogen

Purified virions.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 10mg/ml BSA, 0.01M PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

431

Isotype

IgG1

Research areas

Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza

  • Flow Cytometry - Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC) (ab20921)Flow Cytometry - Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC) (ab20921) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab20921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

Cellular localization

Host cell nucleus

Alternative names

  • Common flu NP antibody
  • Influenza A virus NP antibody
  • NP antibody
  • Nucleocapsid protein antibody
  • Nucleoprotein antibody
  • Protein N antibody
  • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
see all

Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC) images:

  Flow Cytometry - Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC) (ab20921)

Flow Cytometry - Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC) (ab20921)

ab20921 staining Influenza A Virus Nucleoprotein in the Influenza infected Human B-cell lymphoma cell line by Flow Cytometry. Cells were cultured in PBS + 1% BSA and were fixed and permeabilized using a Kit. The sample was incubated with the primary antibody (1/50 in a wash) for 1 hour at 4°C.
Gating Strategy: Single cell suspension.

This image is courtesy of an anonymous Abreview

References for Anti-Influenza A Virus Nucleoprotein antibody [431] (FITC) (ab20921)

This product has been referenced in:

  • Gong Tet al. Recombinant mouse beta-defensin 2 inhibits infection by influenza A virus by blocking its entry. Arch Virol 155:491-8 (2010). ICC/IF; Influenza A.Read more (PubMed: 20195655) »

See 1 publication for this product

Publishing research using ab20921? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"