Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

Overview

  • Product nameAnti-Influenza A Virus Nucleoprotein antibody [AA5H]See all Influenza A Virus Nucleoprotein primary antibodies ...
  • Description
    Mouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein
  • Tested applicationsICC/IF, IHC-P, Flow Cyt, ICCmore details
  • Species reactivity
    Recognises influenza virus A nucleoprotein.
  • Immunogen

    Influenza A/ Puerto Rico/8/34 (H1N1) and A/Bangkok/1/79 (H3N2) viruses.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.5
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notes>95% pure (SDS-PAGE).
  • Clonality Monoclonal
  • Clone numberAA5H
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG2a
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab20343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IHC-P 1/1000.
Flow Cyt Use at an assay dependent concentration. PubMed: 22511783
ICC Use at an assay dependent concentration.

Target

  • RelevanceThe nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • Cellular localizationHost cell nucleus
  • Alternative names
    • Common flu NP antibody
    • Influenza A virus NP antibody
    • NP antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Protein N antibody
    • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
    see all

Anti-Influenza A Virus Nucleoprotein antibody [AA5H] images

  • Ab20343 positively staining formaldehyde fixed Influenza A infected human 293T cells (red) counterstained with AUF-1 (green). ab20343 was used at 1/1000 with no antigen retrieval.

    This image is courtesy of an Abreview submitted by Paul Digard on 24 August 2005. We do not have any further information relating to this image.

    See Abreview

  • ab20343 staining Influenza A Virus Nucleoprotein in porcine lung tissue by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
    Host receptor binding assays with H1N1 classical swine strain (A/Sw/Iowa/15/30), a subtype closely related to the human 1918 pandemic influenza virus. Briefly, paraffin embedded 5 µm sections of lung tissues were deparaffinised in xylene and rehydrated by alcohol. Deparaffinised tissue sections were incubated with TPCK trypsin treated swine influenza virus for 24 hours at 37°C. Paradoxically, we found that mammalian H1N1 virus binds more efficiently at 37°C than at the usual 4°C. The sections were washed, blocked with goat serum for 30 minutes, and incubated with ab20343 at a 1/1000 dilution, overnight in a humidified chamber at 4°C. A secondary antibody, FITC-labelled goat anti-mouse IgG was applied at 1/500 dilution for 2 hours at room temperature. After three further washes with TBS, the sections were mounted with ProLong Gold anti-fade reagent with DAPI.

    Control sections are without virus treatment; low level of auto-fluorescence was detected on alveolar section. 1. alveolar pneumocyte, 2. epithelial lining. Scale bar = 50 µm.
  • ab20343 used at a 1/200 dilution in Flow Cytometry.
    Ubiquitination negatively regulates antiviral activity of IFITM3.

    A and B, HEK293T cells were transfected overnight with indicated plasmids before a 6-hour infection with influenza virus at a multiplicity of infection of 2.5 and analyzed by flow cytometry. Palm? indicates mutation of Cys-71, Cys-72, and Cys-105 to alanine. Ub? indicates mutation of Lys-24, Lys-83, Lys-88, and Lys-104 to alanine. A, cells expressing IFITM3 constructs were analyzed for the percentage of cells that were infected using ab20343. B, antiviral activity was calculated based on the difference in percentage of infection in HA-IFITM3-positive cells compared with vector control with this value set at 100% antiviral activity. Error bars represent the S.D. of triplicate samples. p values were determined using Student's t test. Data are representative of more than five experiments.

References for Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

This product has been referenced in:
  • Liang JJ  et al. Surfactant-modified nanoclay exhibits an antiviral activity with high potency and broad spectrum. J Virol 88:4218-28 (2014). Read more (PubMed: 24478435) »
  • Chesarino NM  et al. Phosphorylation of the antiviral protein interferon-inducible transmembrane protein 3 (IFITM3) dually regulates its endocytosis and ubiquitination. J Biol Chem 289:11986-92 (2014). Flow Cyt . Read more (PubMed: 24627473) »

See all 19 Publications for this product

Product Wall

Thank you for contacting us. Your credit note ID is xxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice ...

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Thank you for taking time to cocontact us and to provide the details of your experiments. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide...

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Thank you for contacting us.

Yes, you will need to dilute the antibody when using in applications e.g. IHC, ICC/IF

Please try concentration10 ug/ml as a start in IHC or ICC/IF.

I hope this information is helpful to you. Pleas...

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Thank you for contacting us.

The product we sell is 200ug per vial. The concentration of lot you have received is 6.87mg/ml which means you should have received a volume of ˜29ul. 4 ul variation could be the pipetting error.

I hope th...

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We have one antibody which may be interesting for you: http://www.abcam.com/index.html?datasheet=20343 (or use the following: http://www.abcam.com/index.html?datasheet=20343). However, please be aware that next to the Puerto Rico (PR)8 strain it ...

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Merci pour votre appel l-autre jour.

Nous sommes désolés que vous avez reçu un tube qui ne contenait pas l'anticorps liquide. Est-ce que après centrifugation vous avez récupérez un peu de liquide? Read More

You will not need to dilute your sample any further after eluting from the beads. As the protocol says, you can after boiling load your sample directly onto your gel, without it affecting anything. If there is anything else I can help you with, please...

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Thanks for your inquiry. The monoclonal antibody to Influenza A Nucleoprotein, catalog ab20343, has been tested and is reactive with the following Influenza A strains: H1N1 A/Puerto Rico/8/34A/USSR/90/77 A/England/333/80 A/Chile/1/83...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Dog Cultured Cells (MDCK)
Specification MDCK
Fixative Methanol
Permeabilization Yes - Acetone
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C
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Verified customer

Submitted Nov 30 2010

Thank you for your inquiry. The laboratory confirmed that they have not tested ab20343 against the intact virus.  The following link is to a published reference that describes the development and original characterizaton of this clone.  The paper in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"