Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

Overview

• Product nameAnti-Influenza A Virus Nucleoprotein antibody [AA5H]See all Influenza A Virus Nucleoprotein primary antibodies ...
• Description
  Mouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein
• Tested applicationsICC/IF, IHC-P, Flow Cyt, ICC more details
• Species reactivity
  Recognises influenza virus A nucleoprotein.
• Immunogen

  Influenza A / Puerto Rico /8/34 (H1N1) and A/Bangkok /1/79 (H3N2) viruses.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS, pH 7.5
• Concentration information loading...
• PurityProtein A purified
• Purification notes>95% pure (SDS-PAGE).
• Clonality Monoclonal
• Clone numberAA5H
• MyelomaP3x63-Ag8.653
• IsotypeIgG2a
• Light chain typeunknown
• Research Areas
  • Microbiology
  • Organism
  • Virus
  • RNA Virus
  • single stranded RNA negative strand virus
  • Influenza

Applications

Target

• RelevanceThe nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
• Cellular localizationHost cell nucleus

• Alternative names
  Common flu NP antibodyInfluenza A virus NP antibodyNP antibody

  Nucleocapsid protein antibodyNucleoprotein antibodyProtein N antibodySeasonal Influenza A (H1N1) Nucleocapsid Protein antibody

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Anti-Influenza A Virus Nucleoprotein antibody [AA5H] images

• 

  Immunocytochemistry - Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

  Ab20343 positively staining formaldehyde fixed Influenza A infected human 293T cells (red) counterstained with AUF-1 (green). ab20343 was used at 1/1000 with no antigen retrieval.
  
  This image is courtesy of an Abreview submitted by Paul Digard on 24 August 2005. We do not have any further information relating to this image.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)Image from Dr RK Nelli at al, BMC Vet Res. 2010 Jan 27;6:4, Fig 5.
  ab20343 staining Influenza A Virus Nucleoprotein in porcine lung tissue by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
  
  Host receptor binding assays with H1N1 classical swine strain (A/Sw/Iowa/15/30), a subtype closely related to the human 1918 pandemic influenza virus. Briefly, paraffin embedded 5 µm sections of lung tissues were deparaffinised in xylene and rehydrated by alcohol. Deparaffinised tissue sections were incubated with TPCK trypsin treated swine influenza virus for 24 hours at 37°C. Paradoxically, we found that mammalian H1N1 virus binds more efficiently at 37°C than at the usual 4°C. The sections were washed, blocked with goat serum for 30 minutes, and incubated with ab20343 at a 1/1000 dilution, overnight in a humidified chamber at 4°C. A secondary antibody, FITC-labelled goat anti-mouse IgG was applied at 1/500 dilution for 2 hours at room temperature. After three further washes with TBS, the sections were mounted with ProLong Gold anti-fade reagent with DAPI.
  
  Control sections are without virus treatment; low level of auto-fluorescence was detected on alveolar section. 1. alveolar pneumocyte, 2. epithelial lining. Scale bar = 50 µm.
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  Flow Cytometry - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)Image from Yount JS et al, J Biol Chem. 2012 Jun 1;287(23):19631-41. Epub 2012 Apr 17, Fig 5. DOI 10.1074/jbc.M112.362095 June 1, 2012 The Journal of Biological Chemistry, 287, 19631-19641.
  ab20343 used at a 1/200 dilution in Flow Cytometry.
  
  Ubiquitination negatively regulates antiviral activity of IFITM3.
  
  A and B, HEK293T cells were transfected overnight with indicated plasmids before a 6-hour infection with influenza virus at a multiplicity of infection of 2.5 and analyzed by flow cytometry. Palm? indicates mutation of Cys-71, Cys-72, and Cys-105 to alanine. Ub? indicates mutation of Lys-24, Lys-83, Lys-88, and Lys-104 to alanine. A, cells expressing IFITM3 constructs were analyzed for the percentage of cells that were infected using ab20343. B, antiviral activity was calculated based on the difference in percentage of infection in HA-IFITM3-positive cells compared with vector control with this value set at 100% antiviral activity. Error bars represent the S.D. of triplicate samples. p values were determined using Student's t test. Data are representative of more than five experiments.