Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
- Product nameAnti-Influenza A Virus Nucleoprotein antibody [AA5H]See all Influenza A Virus Nucleoprotein primary antibodies ...
- DescriptionMouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein
- Tested applicationsICC/IF, IHC-P, Flow Cyt, ICC more details
- Species reactivityRecognises influenza virus A nucleoprotein.
Influenza A / Puerto Rico /8/34 (H1N1) and A/Bangkok /1/79 (H3N2) viruses.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.5
- Concentration information loading...
- PurityProtein A purified
- Purification notes>95% pure (SDS-PAGE).
- Clonality Monoclonal
- Clone numberAA5H
- Light chain typeunknown
- Research Areas
Our Abpromise guarantee covers the use of ab20343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent dilution.|
|Flow Cyt||Flow Cyt: Use at an assay dependent concentration. PubMed: 22511783|
|ICC||ICC: Use at an assay dependent concentration.|
- RelevanceThe nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
- Cellular localizationHost cell nucleus
- Common flu NP antibody
- Influenza A virus NP antibody
- NP antibody
- Nucleocapsid protein antibody
- Nucleoprotein antibody
- Protein N antibody
- Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
Anti-Influenza A Virus Nucleoprotein antibody [AA5H] images
Ab20343 positively staining formaldehyde fixed Influenza A infected human 293T cells (red) counterstained with AUF-1 (green). ab20343 was used at 1/1000 with no antigen retrieval.
This image is courtesy of an Abreview submitted by Paul Digard on 24 August 2005. We do not have any further information relating to this image.
ab20343 staining Influenza A Virus Nucleoprotein in porcine lung tissue by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
Host receptor binding assays with H1N1 classical swine strain (A/Sw/Iowa/15/30), a subtype closely related to the human 1918 pandemic influenza virus. Briefly, paraffin embedded 5 µm sections of lung tissues were deparaffinised in xylene and rehydrated by alcohol. Deparaffinised tissue sections were incubated with TPCK trypsin treated swine influenza virus for 24 hours at 37°C. Paradoxically, we found that mammalian H1N1 virus binds more efficiently at 37°C than at the usual 4°C. The sections were washed, blocked with goat serum for 30 minutes, and incubated with ab20343 at a 1/1000 dilution, overnight in a humidified chamber at 4°C. A secondary antibody, FITC-labelled goat anti-mouse IgG was applied at 1/500 dilution for 2 hours at room temperature. After three further washes with TBS, the sections were mounted with ProLong Gold anti-fade reagent with DAPI.
Control sections are without virus treatment; low level of auto-fluorescence was detected on alveolar section. 1. alveolar pneumocyte, 2. epithelial lining. Scale bar = 50 µm.
ab20343 used at a 1/200 dilution in Flow Cytometry.
Ubiquitination negatively regulates antiviral activity of IFITM3.
A and B, HEK293T cells were transfected overnight with indicated plasmids before a 6-hour infection with influenza virus at a multiplicity of infection of 2.5 and analyzed by flow cytometry. Palm? indicates mutation of Cys-71, Cys-72, and Cys-105 to alanine. Ub? indicates mutation of Lys-24, Lys-83, Lys-88, and Lys-104 to alanine. A, cells expressing IFITM3 constructs were analyzed for the percentage of cells that were infected using ab20343. B, antiviral activity was calculated based on the difference in percentage of infection in HA-IFITM3-positive cells compared with vector control with this value set at 100% antiviral activity. Error bars represent the S.D. of triplicate samples. p values were determined using Student's t test. Data are representative of more than five experiments.
References for Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
This product has been referenced in:
- Sun E et al. Dissecting the role of COPI complexes in influenza virus infection. J Virol 87:2673-85 (2013). Read more (PubMed: 23255804) »
- Hach JC et al. Palmitoylation on conserved and nonconserved cysteines of murine IFITM1 regulates its stability and anti-influenza A virus activity. J Virol 87:9923-7 (2013). Read more (PubMed: 23804635) »