Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

  Immunocytochemistry - Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

Ab20343 positively staining formaldehyde fixed Influenza A infected human 293T cells (red) counterstained with AUF-1 (green). ab20343 was used at 1/1000 with no antigen retrieval.

This image is courtesy of an Abreview submitted by Paul Digard on 24 August 2005. We do not have any further information relating to this image.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

ab20343 staining Influenza A Virus Nucleoprotein in porcine lung tissue by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).

Host receptor binding assays with H1N1 classical swine strain (A/Sw/Iowa/15/30), a subtype closely related to the human 1918 pandemic influenza virus. Briefly, paraffin embedded 5 µm sections of lung tissues were deparaffinised in xylene and rehydrated by alcohol. Deparaffinised tissue sections were incubated with TPCK trypsin treated swine influenza virus for 24 hours at 37°C. Paradoxically, we found that mammalian H1N1 virus binds more efficiently at 37°C than at the usual 4°C. The sections were washed, blocked with goat serum for 30 minutes, and incubated with ab20343 at a 1/1000 dilution, overnight in a humidified chamber at 4°C. A secondary antibody, FITC-labelled goat anti-mouse IgG was applied at 1/500 dilution for 2 hours at room temperature. After three further washes with TBS, the sections were mounted with ProLong Gold anti-fade reagent with DAPI.

Control sections are without virus treatment; low level of auto-fluorescence was detected on alveolar section. 1. alveolar pneumocyte, 2. epithelial lining. Scale bar = 50 µm.

Image from Dr RK Nelli at al, BMC Vet Res. 2010 Jan 27;6:4, Fig 5.