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Products:Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza
Overview
Product name
Anti-Influenza A Virus Nucleoprotein antibody [IV F8]
See all Influenza A Virus Nucleoprotein products (10) ...
Description
Mouse monoclonal [IV F8] to Influenza A Virus Nucleoprotein
Specificity
This antibody reacts with nucleoprotein (NP), which is common for Influenza A(H1N1), A(H3N2) and other Influenza A viruses.
Tested applications
ELISA, ICCmore details
Immunogen
Purified influenza virus type A strain H1N1.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS with 0.1% sodium azide, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is affinity purified.
Clonality
Monoclonal
Clone number
IV F8
Myeloma
Sp2/0
Isotype
IgG1
Light chain type
unknown
Research areas
Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ICC
Application notes
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Can also be used for detection and serotyping of influenza virus type A.
Inhibit viral reproduction after fatty acid acylation.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ICC protocol:
1)Cell monolayer was grown on a glass and infected with the virus for 2 days at 37 oC.
2)Glass was dried and the cells were fixed with cold acetone at 4 oC for 10 min.
3)Ab8261 was introduced in concentrations from 1 to 100 µg/ml in PBS and incubated for 30 min in RT.
4)Glass was washed with PBS several times and incubated with goat anti-mouse IgG labeled with FITC.
Target
Relevance
The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
Cellular localization
Host cell nucleus
Alternative names
- Common flu NP antibody
- Influenza A virus NP antibody
- NP antibody
see all
References for Anti-Influenza A Virus Nucleoprotein antibody [IV F8] (ab8261)
ab8261 has not yet been referenced specifically in any publications.
Publishing research using ab8261? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"