Influenza A Virus Nucleoprotein antibody [IV F8] (ab8261)

Product Name 

Influenza A Virus Nucleoprotein antibody [IV F8]

Product type 

Primary antibodies

Description 

Mouse monoclonal [IV F8] to Influenza A Virus Nucleoprotein

Immunogen 

Purified influenza virus type A strain H1N1.

Specificity 

This antibody reacts with nucleoprotein (NP), which is common for Influenza A(H1N1), A(H3N2) and other Influenza A viruses.

Tested applications 
(see key)

ELISA, ICC

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.

Can also be used for detection and serotyping of influenza virus type A.
Inhibit viral reproduction after fatty acid acylation.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ICC protocol:
1)Cell monolayer was grown on a glass and infected with the virus for 2 days at 37 oC.
2)Glass was dried and the cells were fixed with cold acetone at 4 oC for 10 min.
3)Ab8261 was introduced in concentrations from 1 to 100 µg/ml in PBS and incubated for 30 min in RT.
4)Glass was washed with PBS several times and incubated with goat anti-mouse IgG labeled with FITC.

Cellular localization 

Nuclear

Research areas 

Microbiology >> Organism >> Virus >> RNA Virus >> single stranded RNA negative strand virus >> Influenza

Relevance 

The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1.
"NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

IV F8

Myeloma 

Sp2/0

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

PBS with 0.1% sodium azide, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is affinity purified.

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C.

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