Anti-Insulin + Proinsulin antibody [D6C4] (ab8304)
Key features and details
- Mouse monoclonal [D6C4] to Insulin + Proinsulin
- Suitable for: ELISA
- Reacts with: Mouse, Rat, Cow, Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Insulin + Proinsulin antibody [D6C4]
See all Insulin + Proinsulin primary antibodies -
Description
Mouse monoclonal [D6C4] to Insulin + Proinsulin -
Host species
Mouse -
Specificity
Specific for insulin and proinsulin.
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Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, Pig -
Immunogen
Human insulin.
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General notes
Kd for this antibody is 8.1 x 10-8M.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
Detection of both insulin and proinsulin. -
Clonality
Monoclonal -
Clone number
D6C4 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Affibody® Molecule
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Compatible Secondaries
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Conjugation kits
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Isotype control
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA | (1) |
Use a concentration of 10 µg/ml.
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Notes |
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ELISA
Use a concentration of 10 µg/ml. |
Target
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Relevance
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Defects in insulin are the cause of familial hyperproinsulinemia. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 280829 Cow
- Entrez Gene: 3630 Human
- Entrez Gene: 16333 Mouse
- Entrez Gene: 16334 Mouse
- Entrez Gene: 24505 Rat
- Omim: 176730 Human
- SwissProt: P01317 Cow
- SwissProt: P01308 Human
see all -
Alternative names
- ILPR antibody
- ins antibody
- Insulin antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab8304 has been referenced in 30 publications.
- Southam K et al. Development and characterisation of a rat model that exhibits both metabolic dysfunction and neurodegeneration seen in type 2 diabetes. J Physiol 600:1611-1630 (2022). PubMed: 35128667
- Huang R et al. Retinol-Binding Protein 4 Activates STRA6, Provoking Pancreatic ß-Cell Dysfunction in Type 2 Diabetes. Diabetes 70:449-463 (2021). PubMed: 33199363
- Suthamwong P et al. Administration of mulberry leaves maintains pancreatic ß-cell mass in obese/type 2 diabetes mellitus mouse model. BMC Complement Med Ther 20:136 (2020). PubMed: 32375753
- Viviano J et al. ERp29 as a regulator of Insulin biosynthesis. PLoS One 15:e0233502 (2020). PubMed: 32433667
- Yang SY et al. Prevascularization-free Primary Subcutaneous Transplantation of Xenogeneic Islets Coencapsulated With Hepatocyte Growth Factor. Transplant Direct 6:e620 (2020). PubMed: 33134496