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Product name
Anti-Insulin Receptor R antibody
See all Insulin Receptor R products (4) ...
Description
Rabbit polyclonal to Insulin Receptor R
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 1271-1288 of Human Insulin Receptor R.
Positive control
A257 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Primary antibody notes
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Diabetes
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 144 kDa (predicted molecular weight: 155 kDa).
Target
Function
This receptor probably binds an insulin related protein and has a tyrosine-protein kinase activity. It phosphorylates the insulin receptor substrates IRS-1 and IRS-2.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 3 fibronectin type-III domains.
Contains 1 protein kinase domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionP14616
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- INSRR antibody
- INSRR_HUMAN antibody
- Insulin receptor related receptor precursor antibody
see all
Database links
- Entrez Gene: 3645 Human
- Omim: 147671 Human
- SwissProt: P14616 Human
- Unigene: 248138 Human
Anti-Insulin Receptor R antibody images:
Western blot - Insulin Receptor R antibody (ab5504)
Predicted band size : 155 kDa
ab5504 at a 1/100 dilution staining approximately 144kDa band of Insulin Receptor R in A257 cell lysate by Western blot (ECL). Lane A: preimmune, Lane B: purified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Insulin Receptor R antibody (ab5504)
ab5504 staining Insulin Receptor R in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-Insulin Receptor R antibody (ab5504)
ab5504 has not yet been referenced specifically in any publications.
Publishing research using ab5504? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"