Anti-Insulin antibody [E2E3] (ab9569)
Key features and details
- Mouse monoclonal [E2E3] to Insulin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Insulin antibody [E2E3]
See all Insulin primary antibodies -
Description
Mouse monoclonal [E2E3] to Insulin -
Host species
Mouse -
Specificity
The antibody recognizes the biologically most active forms of insulin on the C terminal end. The antibody labels the cytoplasm of beta cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method). -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Pig Insulin. Full length Native protein
Database link: P01315 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E2E3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Affibody® Molecule
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/100.
Staining of formalin-fixed tissue sections requirestreating in boiling 10mM citrate buffer, pH 6.0 for 10-20 minutes. Primary may be incubated for 60 mins at RT. |
Notes |
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IHC-P
1/100. Staining of formalin-fixed tissue sections requirestreating in boiling 10mM citrate buffer, pH 6.0 for 10-20 minutes. Primary may be incubated for 60 mins at RT. |
Target
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Function
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. -
Involvement in disease
Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3630 Human
- Omim: 176730 Human
- SwissProt: P01308 Human
- Unigene: 272259 Human
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Alternative names
- IDDM antibody
- IDDM1 antibody
- IDDM2 antibody
see all
Images
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Human pancreas stained with ab9569.The antibody labels the cytoplasm of ß cells in pancreatic islands and insulinomas (tested on formalin-fixed, paraffin embedded tissue sections using the Streptavidin-biotinylated peroxidase method).
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Formalin-fixed, paraffin-embedded monkey pancreas tissue stained for Insulin using ab9569 at 1/800 dilution in immunhistochemical analysis.
Goat Ani-mouse HRP was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab9569 has been referenced in 4 publications.
- Guruswamy Damodaran R & Vermette P Decellularized pancreas as a native extracellular matrix scaffold for pancreatic islet seeding and culture. J Tissue Eng Regen Med 12:1230-1237 (2018). PubMed: 29499099
- Enderami SE et al. Generation of Insulin-Producing Cells From Human-Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet-Rich Plasma. J Cell Physiol 232:2878-2886 (2017). PubMed: 27925205
- Tian M et al. Alternative immunomodulatory strategies for xenotransplantation: CD80/CD86-CTLA4 pathway-modified immature dendritic cells promote xenograft survival. PLoS One 8:e69640 (2013). IHC-P . PubMed: 23922766
- Nykiforuk CL et al. Transgenic expression and recovery of biologically active recombinant human insulin from Arabidopsis thaliana seeds. Plant Biotechnol J 4:77-85 (2006). WB ; Human . PubMed: 17177787