Anti-Insulin antibody [K36aC10] (ab6995)
Key features and details
- Mouse monoclonal [K36aC10] to Insulin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Insulin antibody [K36aC10]
See all Insulin primary antibodies -
Description
Mouse monoclonal [K36aC10] to Insulin -
Host species
Mouse -
Specificity
The antibody exhibits cross-reactivity with human proinsulin. This antibody recognizes purified insulin from the pancreas of human, bovine, horse, sheep, and proinsulin from human. Cross reaction has been observed with insulin containing cells in fixed sections of pancreas from human, porcine, dog, rabbit, bovine, sheep, rat, guinea pig and cat. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human insulin.
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Positive control
- IHC: Pancreas tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, BSA -
Concentration information loading...
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Purity
Ascites -
Purification notes
Ascites diluted in a PBS-based diluent. -
Clonality
Monoclonal -
Clone number
K36aC10 -
Isotype
IgG1 -
Research areas
Associated products
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Affibody® Molecule
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (5) |
1/25 - 1/50.
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Notes |
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IHC-P
1/25 - 1/50. |
Target
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Function
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. -
Involvement in disease
Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3630 Human
- Omim: 176730 Human
- SwissProt: P01308 Human
- Unigene: 272259 Human
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Alternative names
- IDDM antibody
- IDDM1 antibody
- IDDM2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (76)
ab6995 has been referenced in 76 publications.
- Sarnobat D et al. Effects of first-line diabetes therapy with biguanides, sulphonylurea and thiazolidinediones on the differentiation, proliferation and apoptosis of islet cell populations. J Endocrinol Invest 45:95-103 (2022). PubMed: 34191257
- Nurliyani N et al. Synbiotic goat milk kefir improves health status in rats fed a high-fat and high-fructose diet. Vet World 15:173-181 (2022). PubMed: 35369595
- Sridhar A et al. Differential effects of RYGB surgery and best medical treatment for obesity-diabetes on intestinal and islet adaptations in obese-diabetic ZDSD rats. PLoS One 17:e0274788 (2022). PubMed: 36137097
- Sarnobat D et al. Effects of artemether on pancreatic islet morphology, islet cell turnover and α-cell transdifferentiation in insulin-deficient GluCreERT2;ROSA26-eYFP diabetic mice. J Pharm Pharmacol 74:1758-1764 (2022). PubMed: 36206181
- Huang CC et al. Risks of Developing Diabetes and Hyperglycemic Crisis Following Carbon Monoxide Poisoning: A Study Incorporating Epidemiologic Analysis and Animal Experiment. Clin Epidemiol 14:1265-1279 (2022). PubMed: 36345392