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- Secondary antibodies
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Product name
Anti-Insulin antibody
See all Insulin products (33) ...
Description
Guinea pig polyclonal to Insulin
Specificity
This antibody stains beta (b) cells of the Langerhan's islets in human pancreas.
Tested applications
IHC-FoFr, ICC/IF, IHC-P, IHC-Fr, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Syrian Hamster, Other
Immunogen
Insulin (Human).
General notes
This product is supplied as Neat Serum. During processing, red blood cells were removed from the blood of the guinea pig. After this, the remaining serum will have a reddish and/or yellowish color, which is added to a clear buffer. Also, there could be fibrin clots, which is sometimes left behind in the purification process. This may cause the antibody to have a cloudy or contaminated appearance, however it is not contaminated and provided with 0.1% sodium azide. Antibody performance is not influenced by this appearance.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Obesity
Metabolism >> Types of disease >> Diabetes
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Insulin antibody (ab7842)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Insulin antibody (ab7842)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Insulin antibody (ab7842)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. PubMed: 20335226
ICC/IF: 1/50 - 1/100.
IHC-P: 1/50 - 1/100. Prolonged fixation in buffered formalin can destroy the epitope.
IHC-Fr: 1/50 - 1/100.
Flow Cyt: Use at an assay dependent concentration. PubMed: 21829502
Target
Relevance
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
Cellular localization
Secreted
Alternative names
- ILPR antibody
- IDDM2 antibody
- INS antibody
see all
Database links
- Entrez Gene: 3630 Human
- Omim: 176730 Human
- SwissProt: P01308 Human
- SwissProt: P01324 Mouse
Anti-Insulin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Insulin antibody (ab7842)
The picture shows beta cells in the pancreas using ab7842 (primary) and ab6906 with Texas Red (secondary). The beta cells in the islets show up very bright red with almost no background.
This image was kindly supplied as part of the review submitted by Kristi Hultman.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Insulin antibody (ab7842)
ab7842 at 1/1000 dilution staining rat pancreas tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The sections were fixed with paraformaldehyde and blocked with 5% serum prior to incubation with the antibody for 12 hours. A Cy2 conjuagted goat polyclonal antibody was used as the secondary. Insulin staining is shown in green. Somatostatin staining is shown in red. DAPI was used as nuclear counterstain.
This image is courtesy of an Abreview submitted by Dr Ulrich Schweizer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Insulin antibody (ab7842)
ab7842 at 1/200 staining mouse pancreas tissue sections by IHC-P.
The tissue section was prepared by Bouin's fixation followed by 1N HCl antigen retrieval and blocked with 1% BSA, 5% goat serum for 4 hours at 20°C.
The primary antibody was incubated for 16 hours at 4°C.
The secondary antibody was a fluorophore labelled Goat anti-Guinea pig polyclonal diluted 1/200.
This image is courtesy of an Abreview submitted by Dr Laura Alonso
Immunohistochemistry (Frozen sections) - Anti-Insulin antibody (ab7842)
ab7842 staining Insulin in human kidney tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in formaldehyde, permeabilized with methanol , blocked using 3% BSA for 1 hour at 22°C then incubated with ab7842 at a 1/200 dilution for 18 hours at 4°C. The secondary used was an Aleza-Fluor 568 conjugated goat anti-guinea pig polyclonal used at a 1/500 dilution.Image shows kidney section bearing RGD-Adv-hHGF-hXIAP-transduced human islets at 30 days after islet transplantation. (a-b) Insulin was stained in red to indicate the functional human islets of mice receiving untransduced human islets (a) and RGD-Adv-hHGF-hXIAP-transduced human islets (b).
This image is courtesy of an anonymous Abreview.
References for Anti-Insulin antibody (ab7842)
This product has been referenced in:
- Dubiel EA et al. In vitro morphogenesis of PANC-1 cells into islet-like aggregates using RGD-covered dextran derivative surfaces. Colloids Surf B Biointerfaces 89:117-25 (2012). IHC-P; Human.Read more (PubMed: 21962947) »
- Yebra M et al. Endothelium-Derived Netrin-4 Supports Pancreatic Epithelial Cell Adhesion and Differentiation through Integrins a2ß1 and a3ß1. PLoS One 6:e22750 (2011). Flow Cyt; Human.Read more (PubMed: 21829502) »
See all 31 publications for this product
Publishing research using ab7842? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"